Development of a Real-Time PCR Assay for the Detection of Donkey (<i>Equus asinus</i>) Meat in Meat Mixtures Treated under Different Processing Conditions

Development of a Real-Time PCR Assay for the Detection of Donkey (<i>Equus asinus</i>) Meat in Meat Mixtures Treated under Different Processing Conditions

In this study, a donkey-specific primer pair and probe were designed from mitochondrial <i>cytochrome b</i> gene for the detection of raw donkey meat and different processed meat mixtures. The PCR product size for donkey DNA was 99 bp, and primer specificity was verified using 20 animal...

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Bibliographic Details
Main Authors: Mi-Ju Kim, Seung-Man Suh, Sung-Yeon Kim, Pei Qin, Hong-Rae Kim, Hae-Yeong Kim
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:Foods
Subjects:
food adulteration
food fraud
donkey
<i>cytochrome b</i>
real-time pcr
meat products
Online Access:https://www.mdpi.com/2304-8158/9/2/130

Internet

https://www.mdpi.com/2304-8158/9/2/130

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