Lipase-catalyzed glycerolysis of fish oil to obtain diacylglycerols

Lipase-catalyzed glycerolysis of fish oil to obtain diacylglycerols

Diacylglycerols (DAG) rich in n-3 residues were successfully produced by Novozym 435-catalysed glycerolysis of n-3 PUFA rich fish oil. Orbital and magnetic agitations were evaluated in order to minimize mass transfer limitations and thus assure the homogeneity of the reactant mixture. Different temp...

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Bibliographic Details
Main Authors: R. Baeza-Jiménez, K. Miranda, H. S. García, C. Otero
Format: Article
Language:English
Published: Consejo Superior de Investigaciones Científicas 2013-06-01
Series:Grasas y Aceites
Subjects:
diacylglycerols
fish oil
glycerolysis
novozym 435
Online Access:http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1428
id doaj-738bf87e44d34eb4af2e080acc60afce
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spelling doaj-738bf87e44d34eb4af2e080acc60afce2021-05-05T07:28:20ZengConsejo Superior de Investigaciones CientíficasGrasas y Aceites0017-34951988-42142013-06-0164323724210.3989/gya.0844121396Lipase-catalyzed glycerolysis of fish oil to obtain diacylglycerolsR. Baeza-Jiménez0K. Miranda1H. S. García2C. Otero3Departamento de Biocatálisis. Instituto de Catálisis y Petroleoquímica, CSICDepartamento de Biocatálisis. Instituto de Catálisis y Petroleoquímica, CSICUNIDA, Instituto Tecnológico de VeracruzDepartamento de Biocatálisis. Instituto de Catálisis y Petroleoquímica, CSICDiacylglycerols (DAG) rich in n-3 residues were successfully produced by Novozym 435-catalysed glycerolysis of n-3 PUFA rich fish oil. Orbital and magnetic agitations were evaluated in order to minimize mass transfer limitations and thus assure the homogeneity of the reactant mixture. Different temperatures (65, 70, 75, 80, 85 and 90 °C) and speeds (300, 500, 700 and 900 rpm) were tested. Optimal conditions to obtain the highest amount of DAG were: 65 °C, with a substrate molar ratio of 3:1 (oil:glycerol), 500 rpm and 15% enzyme load after 2.5 h, with a yield of 60%.http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1428diacylglycerolsfish oilglycerolysisnovozym 435
collection DOAJ
language English
format Article
sources DOAJ
author R. Baeza-Jiménez
K. Miranda
H. S. García
C. Otero
spellingShingle R. Baeza-Jiménez
K. Miranda
H. S. García
C. Otero
Lipase-catalyzed glycerolysis of fish oil to obtain diacylglycerols
Grasas y Aceites
diacylglycerols
fish oil
glycerolysis
novozym 435
author_facet R. Baeza-Jiménez
K. Miranda
H. S. García
C. Otero
author_sort R. Baeza-Jiménez
title Lipase-catalyzed glycerolysis of fish oil to obtain diacylglycerols
title_short Lipase-catalyzed glycerolysis of fish oil to obtain diacylglycerols
title_full Lipase-catalyzed glycerolysis of fish oil to obtain diacylglycerols
title_fullStr Lipase-catalyzed glycerolysis of fish oil to obtain diacylglycerols
title_full_unstemmed Lipase-catalyzed glycerolysis of fish oil to obtain diacylglycerols
title_sort lipase-catalyzed glycerolysis of fish oil to obtain diacylglycerols
publisher Consejo Superior de Investigaciones Científicas
series Grasas y Aceites
issn 0017-3495
1988-4214
publishDate 2013-06-01
description Diacylglycerols (DAG) rich in n-3 residues were successfully produced by Novozym 435-catalysed glycerolysis of n-3 PUFA rich fish oil. Orbital and magnetic agitations were evaluated in order to minimize mass transfer limitations and thus assure the homogeneity of the reactant mixture. Different temperatures (65, 70, 75, 80, 85 and 90 °C) and speeds (300, 500, 700 and 900 rpm) were tested. Optimal conditions to obtain the highest amount of DAG were: 65 °C, with a substrate molar ratio of 3:1 (oil:glycerol), 500 rpm and 15% enzyme load after 2.5 h, with a yield of 60%.
topic diacylglycerols
fish oil
glycerolysis
novozym 435
url http://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/1428
work_keys_str_mv AT rbaezajimenez lipasecatalyzedglycerolysisoffishoiltoobtaindiacylglycerols
AT kmiranda lipasecatalyzedglycerolysisoffishoiltoobtaindiacylglycerols
AT hsgarcia lipasecatalyzedglycerolysisoffishoiltoobtaindiacylglycerols
AT cotero lipasecatalyzedglycerolysisoffishoiltoobtaindiacylglycerols
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