Using cryo-electron microscopy maps for X-ray structure determination
X-ray crystallography and cryo-electron microscopy (cryo-EM) are complementary techniques for structure determination. Crystallography usually reveals more detailed information, while cryo-EM is an extremely useful technique for studying large-sized macromolecules. As the gap between the resolution...
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doaj-736d7db4d2724fea800ffd5ff909aa082020-11-25T01:06:42ZengInternational Union of CrystallographyIUCrJ2052-25252018-07-015438238910.1107/S2052252518005857cw5016Using cryo-electron microscopy maps for X-ray structure determinationLingxiao Zeng0Wei Ding1Quan Hao2School of Biomedical Sciences, University of Hong Kong, 21 Sassoon Road, Hong KongInstitute of Physics, Chinese Academy of Sciences, Beijing 100190, People's Republic of ChinaSchool of Biomedical Sciences, University of Hong Kong, 21 Sassoon Road, Hong KongX-ray crystallography and cryo-electron microscopy (cryo-EM) are complementary techniques for structure determination. Crystallography usually reveals more detailed information, while cryo-EM is an extremely useful technique for studying large-sized macromolecules. As the gap between the resolution of crystallography and cryo-EM data narrows, the cryo-EM map of a macromolecule could serve as an initial model to solve the phase problem of crystal diffraction for high-resolution structure determination. FSEARCH is a procedure to utilize the low-resolution molecular shape for crystallographic phasing. The IPCAS (Iterative Protein Crystal structure Automatic Solution) pipeline is an automatic direct-methods-aided dual-space iterative phasing and model-building procedure. When only an electron-density map is available as the starting point, IPCAS is capable of generating a completed model from the phases of the input map automatically, without the requirement of an initial model. In this study, a hybrid method integrating X-ray crystallography with cryo-EM to help with structure determination is presented. With a cryo-EM map as the starting point, the workflow of the method involves three steps. (1) Cryo-EM map replacement: FSEARCH is utilized to find the correct translation and orientation of the cryo-EM map in the crystallographic unit cell and generates the initial low-resolution map. (2) Phase extension: the phases calculated from the correctly placed cryo-EM map are extended to high-resolution X-ray data by non-crystallographic symmetry averaging with phenix.resolve. (3) Model building: IPCAS is used to generate an initial model using the phase-extended map and perform model completion by iteration. Four cases (the lowest cryo-EM map resolution being 6.9 Å) have been tested for the general applicability of the hybrid method, and almost complete models have been generated for all test cases with reasonable Rwork/Rfree. The hybrid method therefore provides an automated tool for X-ray structure determination using a cryo-EM map as the starting point.http://scripts.iucr.org/cgi-bin/paper?S2052252518005857FSEARCHIPCASiterative phasingcryo-EMmodel buildingstructure determinationcryo-electron microscopyX-ray crystallographyphase problem |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lingxiao Zeng Wei Ding Quan Hao |
spellingShingle |
Lingxiao Zeng Wei Ding Quan Hao Using cryo-electron microscopy maps for X-ray structure determination IUCrJ FSEARCH IPCAS iterative phasing cryo-EM model building structure determination cryo-electron microscopy X-ray crystallography phase problem |
author_facet |
Lingxiao Zeng Wei Ding Quan Hao |
author_sort |
Lingxiao Zeng |
title |
Using cryo-electron microscopy maps for X-ray structure determination |
title_short |
Using cryo-electron microscopy maps for X-ray structure determination |
title_full |
Using cryo-electron microscopy maps for X-ray structure determination |
title_fullStr |
Using cryo-electron microscopy maps for X-ray structure determination |
title_full_unstemmed |
Using cryo-electron microscopy maps for X-ray structure determination |
title_sort |
using cryo-electron microscopy maps for x-ray structure determination |
publisher |
International Union of Crystallography |
series |
IUCrJ |
issn |
2052-2525 |
publishDate |
2018-07-01 |
description |
X-ray crystallography and cryo-electron microscopy (cryo-EM) are complementary techniques for structure determination. Crystallography usually reveals more detailed information, while cryo-EM is an extremely useful technique for studying large-sized macromolecules. As the gap between the resolution of crystallography and cryo-EM data narrows, the cryo-EM map of a macromolecule could serve as an initial model to solve the phase problem of crystal diffraction for high-resolution structure determination. FSEARCH is a procedure to utilize the low-resolution molecular shape for crystallographic phasing. The IPCAS (Iterative Protein Crystal structure Automatic Solution) pipeline is an automatic direct-methods-aided dual-space iterative phasing and model-building procedure. When only an electron-density map is available as the starting point, IPCAS is capable of generating a completed model from the phases of the input map automatically, without the requirement of an initial model. In this study, a hybrid method integrating X-ray crystallography with cryo-EM to help with structure determination is presented. With a cryo-EM map as the starting point, the workflow of the method involves three steps. (1) Cryo-EM map replacement: FSEARCH is utilized to find the correct translation and orientation of the cryo-EM map in the crystallographic unit cell and generates the initial low-resolution map. (2) Phase extension: the phases calculated from the correctly placed cryo-EM map are extended to high-resolution X-ray data by non-crystallographic symmetry averaging with phenix.resolve. (3) Model building: IPCAS is used to generate an initial model using the phase-extended map and perform model completion by iteration. Four cases (the lowest cryo-EM map resolution being 6.9 Å) have been tested for the general applicability of the hybrid method, and almost complete models have been generated for all test cases with reasonable Rwork/Rfree. The hybrid method therefore provides an automated tool for X-ray structure determination using a cryo-EM map as the starting point. |
topic |
FSEARCH IPCAS iterative phasing cryo-EM model building structure determination cryo-electron microscopy X-ray crystallography phase problem |
url |
http://scripts.iucr.org/cgi-bin/paper?S2052252518005857 |
work_keys_str_mv |
AT lingxiaozeng usingcryoelectronmicroscopymapsforxraystructuredetermination AT weiding usingcryoelectronmicroscopymapsforxraystructuredetermination AT quanhao usingcryoelectronmicroscopymapsforxraystructuredetermination |
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