Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study
Demand for microbial colorants is now becoming a competitive research topic for food, cosmetics and pharmaceutics industries. In most applications, the pigments of interest such as polyketide-based red pigments from fungal submerged cultures are extracted by conventional liquid–liquid extraction met...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-12-01
|
Series: | Journal of Fungi |
Subjects: | |
Online Access: | https://www.mdpi.com/2309-608X/6/4/375 |
id |
doaj-734d10a6f5f9438995bfd75b7658bd32 |
---|---|
record_format |
Article |
spelling |
doaj-734d10a6f5f9438995bfd75b7658bd322020-12-19T00:00:38ZengMDPI AGJournal of Fungi2309-608X2020-12-01637537510.3390/jof6040375Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case StudyJuliana Lebeau0Thomas Petit1Mireille Fouillaud2Laurent Dufossé3Yanis Caro4Laboratoire de Chimie et de Biotechnologie des Produits Naturels (CHEMBIOPRO), Université de La Réunion, F-97490 Sainte-Clotilde, FranceLaboratoire de Chimie et de Biotechnologie des Produits Naturels (CHEMBIOPRO), Université de La Réunion, F-97490 Sainte-Clotilde, FranceLaboratoire de Chimie et de Biotechnologie des Produits Naturels (CHEMBIOPRO), Université de La Réunion, F-97490 Sainte-Clotilde, FranceLaboratoire de Chimie et de Biotechnologie des Produits Naturels (CHEMBIOPRO), Université de La Réunion, F-97490 Sainte-Clotilde, FranceLaboratoire de Chimie et de Biotechnologie des Produits Naturels (CHEMBIOPRO), Université de La Réunion, F-97490 Sainte-Clotilde, FranceDemand for microbial colorants is now becoming a competitive research topic for food, cosmetics and pharmaceutics industries. In most applications, the pigments of interest such as polyketide-based red pigments from fungal submerged cultures are extracted by conventional liquid–liquid extraction methods requiring large volumes of various organic solvents and time. To address this question from a different angle, we proposed, here, to investigate the use of three different aqueous two-phase extraction systems using either ammonium- or imidazolium-based ionic liquids. We applied these to four fermentation broths of <i>Talaromyces albobiverticillius</i> (deep red pigment producer), <i>Emericella purpurea</i> (red pigment producer), <i>Paecilomyces marquandii</i> (yellow pigment producer) and <i>Trichoderma harzianum</i> (yellow-brown pigment producer) to investigate their selective extraction abilities towards the detection of polyketide-based pigments. Our findings led us to conclude that (i) these alternative extraction systems using ionic liquids as greener extractant means worked well for this extraction of colored molecules from the fermentation broths of the filamentous fungi investigated here; (ii) tetrabutylammonium bromide, [N4444]Br-, showed the best pigment extraction ability, with a higher putative affinity for azaphilone red pigments; (iii) the back extraction and recovery of the fungal pigments from ionic liquid phases remained the limiting point of the method under our selected conditions for potential industrial applications. Nevertheless, these alternative extraction procedures appeared to be promising ways for the detection of polyketide-based colorants in the submerged cultures of filamentous fungi.https://www.mdpi.com/2309-608X/6/4/375fungal pigmentnatural colorantextraction abilitymarine fungi<i>Talaromyces albobiverticillius</i>aqueous two-phases system extraction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juliana Lebeau Thomas Petit Mireille Fouillaud Laurent Dufossé Yanis Caro |
spellingShingle |
Juliana Lebeau Thomas Petit Mireille Fouillaud Laurent Dufossé Yanis Caro Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study Journal of Fungi fungal pigment natural colorant extraction ability marine fungi <i>Talaromyces albobiverticillius</i> aqueous two-phases system extraction |
author_facet |
Juliana Lebeau Thomas Petit Mireille Fouillaud Laurent Dufossé Yanis Caro |
author_sort |
Juliana Lebeau |
title |
Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study |
title_short |
Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study |
title_full |
Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study |
title_fullStr |
Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study |
title_full_unstemmed |
Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study |
title_sort |
aqueous two-phase system extraction of polyketide-based fungal pigments using ammonium- or imidazolium-based ionic liquids for detection purpose: a case study |
publisher |
MDPI AG |
series |
Journal of Fungi |
issn |
2309-608X |
publishDate |
2020-12-01 |
description |
Demand for microbial colorants is now becoming a competitive research topic for food, cosmetics and pharmaceutics industries. In most applications, the pigments of interest such as polyketide-based red pigments from fungal submerged cultures are extracted by conventional liquid–liquid extraction methods requiring large volumes of various organic solvents and time. To address this question from a different angle, we proposed, here, to investigate the use of three different aqueous two-phase extraction systems using either ammonium- or imidazolium-based ionic liquids. We applied these to four fermentation broths of <i>Talaromyces albobiverticillius</i> (deep red pigment producer), <i>Emericella purpurea</i> (red pigment producer), <i>Paecilomyces marquandii</i> (yellow pigment producer) and <i>Trichoderma harzianum</i> (yellow-brown pigment producer) to investigate their selective extraction abilities towards the detection of polyketide-based pigments. Our findings led us to conclude that (i) these alternative extraction systems using ionic liquids as greener extractant means worked well for this extraction of colored molecules from the fermentation broths of the filamentous fungi investigated here; (ii) tetrabutylammonium bromide, [N4444]Br-, showed the best pigment extraction ability, with a higher putative affinity for azaphilone red pigments; (iii) the back extraction and recovery of the fungal pigments from ionic liquid phases remained the limiting point of the method under our selected conditions for potential industrial applications. Nevertheless, these alternative extraction procedures appeared to be promising ways for the detection of polyketide-based colorants in the submerged cultures of filamentous fungi. |
topic |
fungal pigment natural colorant extraction ability marine fungi <i>Talaromyces albobiverticillius</i> aqueous two-phases system extraction |
url |
https://www.mdpi.com/2309-608X/6/4/375 |
work_keys_str_mv |
AT julianalebeau aqueoustwophasesystemextractionofpolyketidebasedfungalpigmentsusingammoniumorimidazoliumbasedionicliquidsfordetectionpurposeacasestudy AT thomaspetit aqueoustwophasesystemextractionofpolyketidebasedfungalpigmentsusingammoniumorimidazoliumbasedionicliquidsfordetectionpurposeacasestudy AT mireillefouillaud aqueoustwophasesystemextractionofpolyketidebasedfungalpigmentsusingammoniumorimidazoliumbasedionicliquidsfordetectionpurposeacasestudy AT laurentdufosse aqueoustwophasesystemextractionofpolyketidebasedfungalpigmentsusingammoniumorimidazoliumbasedionicliquidsfordetectionpurposeacasestudy AT yaniscaro aqueoustwophasesystemextractionofpolyketidebasedfungalpigmentsusingammoniumorimidazoliumbasedionicliquidsfordetectionpurposeacasestudy |
_version_ |
1724378216223735808 |