Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study

Demand for microbial colorants is now becoming a competitive research topic for food, cosmetics and pharmaceutics industries. In most applications, the pigments of interest such as polyketide-based red pigments from fungal submerged cultures are extracted by conventional liquid–liquid extraction met...

Full description

Bibliographic Details
Main Authors: Juliana Lebeau, Thomas Petit, Mireille Fouillaud, Laurent Dufossé, Yanis Caro
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:Journal of Fungi
Subjects:
Online Access:https://www.mdpi.com/2309-608X/6/4/375
id doaj-734d10a6f5f9438995bfd75b7658bd32
record_format Article
spelling doaj-734d10a6f5f9438995bfd75b7658bd322020-12-19T00:00:38ZengMDPI AGJournal of Fungi2309-608X2020-12-01637537510.3390/jof6040375Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case StudyJuliana Lebeau0Thomas Petit1Mireille Fouillaud2Laurent Dufossé3Yanis Caro4Laboratoire de Chimie et de Biotechnologie des Produits Naturels (CHEMBIOPRO), Université de La Réunion, F-97490 Sainte-Clotilde, FranceLaboratoire de Chimie et de Biotechnologie des Produits Naturels (CHEMBIOPRO), Université de La Réunion, F-97490 Sainte-Clotilde, FranceLaboratoire de Chimie et de Biotechnologie des Produits Naturels (CHEMBIOPRO), Université de La Réunion, F-97490 Sainte-Clotilde, FranceLaboratoire de Chimie et de Biotechnologie des Produits Naturels (CHEMBIOPRO), Université de La Réunion, F-97490 Sainte-Clotilde, FranceLaboratoire de Chimie et de Biotechnologie des Produits Naturels (CHEMBIOPRO), Université de La Réunion, F-97490 Sainte-Clotilde, FranceDemand for microbial colorants is now becoming a competitive research topic for food, cosmetics and pharmaceutics industries. In most applications, the pigments of interest such as polyketide-based red pigments from fungal submerged cultures are extracted by conventional liquid–liquid extraction methods requiring large volumes of various organic solvents and time. To address this question from a different angle, we proposed, here, to investigate the use of three different aqueous two-phase extraction systems using either ammonium- or imidazolium-based ionic liquids. We applied these to four fermentation broths of <i>Talaromyces albobiverticillius</i> (deep red pigment producer), <i>Emericella purpurea</i> (red pigment producer), <i>Paecilomyces marquandii</i> (yellow pigment producer) and <i>Trichoderma harzianum</i> (yellow-brown pigment producer) to investigate their selective extraction abilities towards the detection of polyketide-based pigments. Our findings led us to conclude that (i) these alternative extraction systems using ionic liquids as greener extractant means worked well for this extraction of colored molecules from the fermentation broths of the filamentous fungi investigated here; (ii) tetrabutylammonium bromide, [N4444]Br-, showed the best pigment extraction ability, with a higher putative affinity for azaphilone red pigments; (iii) the back extraction and recovery of the fungal pigments from ionic liquid phases remained the limiting point of the method under our selected conditions for potential industrial applications. Nevertheless, these alternative extraction procedures appeared to be promising ways for the detection of polyketide-based colorants in the submerged cultures of filamentous fungi.https://www.mdpi.com/2309-608X/6/4/375fungal pigmentnatural colorantextraction abilitymarine fungi<i>Talaromyces albobiverticillius</i>aqueous two-phases system extraction
collection DOAJ
language English
format Article
sources DOAJ
author Juliana Lebeau
Thomas Petit
Mireille Fouillaud
Laurent Dufossé
Yanis Caro
spellingShingle Juliana Lebeau
Thomas Petit
Mireille Fouillaud
Laurent Dufossé
Yanis Caro
Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study
Journal of Fungi
fungal pigment
natural colorant
extraction ability
marine fungi
<i>Talaromyces albobiverticillius</i>
aqueous two-phases system extraction
author_facet Juliana Lebeau
Thomas Petit
Mireille Fouillaud
Laurent Dufossé
Yanis Caro
author_sort Juliana Lebeau
title Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study
title_short Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study
title_full Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study
title_fullStr Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study
title_full_unstemmed Aqueous Two-Phase System Extraction of Polyketide-Based Fungal Pigments Using Ammonium- or Imidazolium-Based Ionic Liquids for Detection Purpose: A Case Study
title_sort aqueous two-phase system extraction of polyketide-based fungal pigments using ammonium- or imidazolium-based ionic liquids for detection purpose: a case study
publisher MDPI AG
series Journal of Fungi
issn 2309-608X
publishDate 2020-12-01
description Demand for microbial colorants is now becoming a competitive research topic for food, cosmetics and pharmaceutics industries. In most applications, the pigments of interest such as polyketide-based red pigments from fungal submerged cultures are extracted by conventional liquid–liquid extraction methods requiring large volumes of various organic solvents and time. To address this question from a different angle, we proposed, here, to investigate the use of three different aqueous two-phase extraction systems using either ammonium- or imidazolium-based ionic liquids. We applied these to four fermentation broths of <i>Talaromyces albobiverticillius</i> (deep red pigment producer), <i>Emericella purpurea</i> (red pigment producer), <i>Paecilomyces marquandii</i> (yellow pigment producer) and <i>Trichoderma harzianum</i> (yellow-brown pigment producer) to investigate their selective extraction abilities towards the detection of polyketide-based pigments. Our findings led us to conclude that (i) these alternative extraction systems using ionic liquids as greener extractant means worked well for this extraction of colored molecules from the fermentation broths of the filamentous fungi investigated here; (ii) tetrabutylammonium bromide, [N4444]Br-, showed the best pigment extraction ability, with a higher putative affinity for azaphilone red pigments; (iii) the back extraction and recovery of the fungal pigments from ionic liquid phases remained the limiting point of the method under our selected conditions for potential industrial applications. Nevertheless, these alternative extraction procedures appeared to be promising ways for the detection of polyketide-based colorants in the submerged cultures of filamentous fungi.
topic fungal pigment
natural colorant
extraction ability
marine fungi
<i>Talaromyces albobiverticillius</i>
aqueous two-phases system extraction
url https://www.mdpi.com/2309-608X/6/4/375
work_keys_str_mv AT julianalebeau aqueoustwophasesystemextractionofpolyketidebasedfungalpigmentsusingammoniumorimidazoliumbasedionicliquidsfordetectionpurposeacasestudy
AT thomaspetit aqueoustwophasesystemextractionofpolyketidebasedfungalpigmentsusingammoniumorimidazoliumbasedionicliquidsfordetectionpurposeacasestudy
AT mireillefouillaud aqueoustwophasesystemextractionofpolyketidebasedfungalpigmentsusingammoniumorimidazoliumbasedionicliquidsfordetectionpurposeacasestudy
AT laurentdufosse aqueoustwophasesystemextractionofpolyketidebasedfungalpigmentsusingammoniumorimidazoliumbasedionicliquidsfordetectionpurposeacasestudy
AT yaniscaro aqueoustwophasesystemextractionofpolyketidebasedfungalpigmentsusingammoniumorimidazoliumbasedionicliquidsfordetectionpurposeacasestudy
_version_ 1724378216223735808