The Comparison of Efficiency of Density Gradient Centrifugation and Zeta Methods in Separation of Mature Sperm with Normal Chromatin Structure

The Comparison of Efficiency of Density Gradient Centrifugation and Zeta Methods in Separation of Mature Sperm with Normal Chromatin Structure

Objective: To examine the efficiency of both the Zeta and density gradient centrifugation(DGC) methods for the selection of normal chromatin sperm by TUNEL, sperm chromatindispersion (SCD), acridine orange and chromomycin A3 (CMA3).Materials and Methods: The study was performed on 63 patients. Semen...

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Bibliographic Details
Main Authors: Mohammad Hossein Nasr Esfahani, Shahnaz Razavi, Mohammad Mardani, Noush Afarin Khajavi, Mohammad Reza Deemeh, Marziyeh Tavalaee
Format: Article
Language:English
Published: Royan Institute (ACECR), Tehran 2009-01-01
Series:Cell Journal
Subjects:
Zeta Method
Protamine
Morphology
Sperm
DNA Fragmentation
Online Access:http://celljournal.org/library/upload/article/Article9.pdf

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