| Summary: | The aim of this study was to characterize staphylococci and streptococci in milk from Egyptian bovides. In total, 50 milk samples were collected from localities in the Nile Delta region of Egypt. Isolates were cultivated, identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and antibiotic susceptibility testing was performed by the broth microdilution method. PCR amplifications were carried out, targeting resistance-associated genes. Thirty-eight <i>Staphylococcus</i> isolates and six<i> Streptococcus</i> isolates could be cultivated.<i> Staphylococcus aureus</i> isolates revealed a high resistance rate to penicillin, ampicillin, clindamycin, and erythromycin. The <i>mec</i>A gene defining methicillin-resistant <i>Staphylococcus aureus</i>, <i>erm</i>(C) and<i> aac-aph</i>D genes was found in 87.5% of each. Coagulase-negative staphylococci showed a high prevalence of <i>mec</i>A, <i>bla</i>Z and <i>tet</i>K genes. Other resistance-associated genes were found. All <i>Streptococcus dysgalactiae</i> isolates carried <i>bla</i>Z, <i>erm</i>(A), <i>erm</i>(B), <i>erm</i>(C) and <i>lnu</i>A genes, while <i>Streptococcus suis</i> harbored <i>erm</i>(C), <i>aph</i>A-3<i>, tet</i>L and <i>tet</i>M genes, additionally. In <i>Streptococcus gallolyticus</i>, most of these genes were found. The <i>Streptococcus agalactiae</i> isolate harbored <i>bla</i>Z, <i>erm</i>(B), <i>erm</i>(C), <i>lnu</i>A, <i>tet</i>K, <i>tet</i>L and <i>tet</i>M genes. <i>Streptococcus</i> <i>agalactiae </i>isolate was analyzed by DNA microarray analysis. It was determined as sequence type 14, belonging to clonal complex 19 and represented capsule type VI. Pilus and cell wall protein genes, <i>pav</i>A, <i>cad</i>D and <i>emr</i>B/<i>qac</i>A genes were identified by microarray analysis.
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