snRNP: Rich Nuclear Bodies in Hyacinthus orientalis L. Microspores and Developing Pollen Cells
The aim of the present work was the characterization of nuclear bodies in the microspore and developing pollen cells of Hyacinthus orientalis L.. The combination of Ag-NOR, immunofluorescence and immunogold techniques was used in this study. The obtained results showed the presence of highly agyroph...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi Limited
2009-01-01
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| Series: | International Journal of Cell Biology |
| Online Access: | http://dx.doi.org/10.1155/2009/209303 |
| Summary: | The aim of the present work was the characterization of nuclear bodies in the microspore and developing pollen cells of Hyacinthus orientalis L.. The combination of Ag-NOR, immunofluorescence and immunogold techniques was used in this study. The obtained results showed the presence of highly agyrophylic extranucleolar bodies in microspore and developing pollen cells, which were finally identified
as Cajal bodies. In all cases, a strong accumulation of snRNP-indicating molecules including TMG cap, Sm
proteins and U2 snRNA, was observed in the examined nuclear bodies. In contrast to their number the
size of the identified structures did not change significantly during pollen development. In the microspore
and the vegetative cell of pollen grains CBs were more numerous than in the generative cell. At later
stages of pollen development, a drastic decrease in CB number was observed and, just before anthesis, a
complete lack of these structures was indicated in both pollen nuclei. On the basis of these results, as well as our previous studies, we postulate a strong relationship between Cajal body numbers and the levels of
RNA synthesis and splicing machinery elements in microspore and developing pollen cells. |
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| ISSN: | 1687-8876 1687-8884 |