Myelin enhancement of Multiple sclerosis model with gold nanoparticles into the corpus callosum

• Main Authors: Mahjabin Khosravi, Manizheh Karami, Mohammadreza Jalali Nadoushan, Abazar Hajnorouzi
• Format: Article
• Language: English
• Published: Mashhad University of Medical Sciences 2019-10-01
• Series: Nanomedicine Journal
• Subjects: corpus callosum; demyelination; gold nanoparticle; l-arginine; multiple sclerosis; nitric oxide; rat
• Online Access: http://nmj.mums.ac.ir/article_13308_8ea0dae7d856531e1834ac7c1a7f333b.pdf

Objective(s): With no substantial cost, we injected L-arginine into the rat’s corpus callosum (CC) to create animal model of multiple sclerosis (MS) and investigated the pre-injection effect of gold nanoparticles (GNPs). <br />Materials and Methods: Adult male Wistar rat (250-300 g) was surgically cannulated at the CC, and after recovery it was injected L-arginine (3-200 µg/rat, intra-CC) once daily for 3 to 5 consecutive days. GNPs (0.001-0.01 µg/rat, intra-CC) were injected alone or prior to the L-arginine using the same procedure. Control group solely received saline (1 µL/rat, intra-CC). Brain was studied with luxol fast blue. Weight change was also analyzed via the analysis of variance (ANOVA). <br />Results: L-arginine significantly induced (p< 0.05) a reduction in the fiber density while the neurons increased (p< 0.05). Single GNPs reduced (p< 0.05) the fiber and neuron densities; however, pre-injection of NPs caused myelinated fibers and uniform density of neurons. <br />Conclusion: The L-arginine may trigger demyelination by pro-inflammatory nitric oxide (NO), and the GNPs may improve this effect.