Serially transplantable mammary epithelial cells express the Thy-1 antigen

• Main Authors: Neethan Amit Lobo, Maider Zabala, Dalong Qian, Michael F. Clarke
• Format: Article
• Language: English
• Published: BMC 2018-10-01
• Series: Breast Cancer Research
• Subjects: Tissue-specific stem cells; In vivo serial transplantation; Progenitor cells; Self-renewal
• Online Access: http://link.springer.com/article/10.1186/s13058-018-1006-y

Abstract Background Recent studies in murine mammary tissue have identified functionally distinct cell populations that may be isolated by surface phenotype or lineage tracing. Previous groups have shown that CD24medCD49fhigh cells enriched for long-lived mammary epithelial cells can be serially transplanted. Methods Flow cytometry-based enrichment of distinct phenotypic populations was assessed for their gene expression profiles and functional proliferative attributes in vitro and in vivo. Results Here, we show Thy-1 is differentially expressed in the CD24medCD49fhigh population, which allowed us to discern two functionally different populations. The Thy-1+CD24medCD49fhigh phenotype contained the majority of the serially transplantable epithelial cells. The Thy-1−CD24medCD49fhigh phenotype contains a rare progenitor population that is able to form primary mammary outgrowths with significantly decreased serial in vivo transplantation potential. Conclusions Therefore, Thy-1 expression in the immature cell compartment is a useful tool to study the functional heterogeneity that drives mammary gland development and has implications for disease etiology.