Active Expression of Membrane-Bound L-Amino Acid Deaminase from <i>Proteus mirabilis</i> in Recombinant <i>Escherichia coli</i> by Fusion with Maltose-Binding Protein for Enhanced Catalytic Performance

Active Expression of Membrane-Bound L-Amino Acid Deaminase from <i>Proteus mirabilis</i> in Recombinant <i>Escherichia coli</i> by Fusion with Maltose-Binding Protein for Enhanced Catalytic Performance

L-amino acid deaminases (LAADs) are membrane flavoenzymes that catalyze the deamination of neutral and aromatic L-amino acids to &#945;-keto acids and ammonia. LAADs can be used to develop many important biotechnological applications. However, the transmembrane &#945;-helix of LAADs restrict...

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Bibliographic Details
Main Authors: Dan-Ping Zhang, Xiao-Ran Jing, An-Wen Fan, Huan Liu, Yao Nie, Yan Xu
Format: Article
Language:English
Published: MDPI AG 2020-02-01
Series:Catalysts
Subjects:
l-amino acid deaminases
membrane-bound protein
fusion protein
soluble expression
maltose-binding protein tag
Online Access:https://www.mdpi.com/2073-4344/10/2/215

Internet

https://www.mdpi.com/2073-4344/10/2/215

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