Pulsatile Flow-Induced Fatigue-Resistant Photopolymerizable Hydrogels for the Treatment of Intracranial Aneurysms

An alternative intracranial aneurysm embolic agent is emerging in the form of hydrogels due to their ability to be injected in liquid phase and solidify in situ. Hydrogels have the ability to fill an aneurysm sac more completely compared to solid implants such as those used in coil embolization. Rec...

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Main Authors: Oriane Poupart, Riccardo Conti, Andreas Schmocker, Lucio Pancaldi, Christophe Moser, Katja M. Nuss, Mahmut S. Sakar, Tomas Dobrocky, Hansjörg Grützmacher, Pascal J. Mosimann, Dominique P. Pioletti
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-01-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2020.619858/full
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spelling doaj-72240637e57941149a78783b4ce054b42021-01-20T06:40:46ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-01-01810.3389/fbioe.2020.619858619858Pulsatile Flow-Induced Fatigue-Resistant Photopolymerizable Hydrogels for the Treatment of Intracranial AneurysmsOriane Poupart0Riccardo Conti1Andreas Schmocker2Andreas Schmocker3Andreas Schmocker4Lucio Pancaldi5Christophe Moser6Katja M. Nuss7Mahmut S. Sakar8Tomas Dobrocky9Hansjörg Grützmacher10Pascal J. Mosimann11Pascal J. Mosimann12Dominique P. Pioletti13Laboratory of Biomechanical Orthopedics, École Polytechnique Fédérale de Lausanne, Lausanne, SwitzerlandDepartment of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, Zurich, SwitzerlandDepartment of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, Zurich, SwitzerlandLaboratory of Applied Photonics Devices, École Polytechnique Fédérale de Lausanne, Lausanne, SwitzerlandInstitute of Diagnostic and Interventional Neuroradiology, Inselspital, Bern University Hospital, Bern, SwitzerlandInstitute of Mechanical Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, SwitzerlandLaboratory of Applied Photonics Devices, École Polytechnique Fédérale de Lausanne, Lausanne, SwitzerlandMusculoskeletal Research Unit, Department of Molecular Mechanisms of Disease, Vetsuisse Faculty, University of Zurich, Zurich, SwitzerlandInstitute of Mechanical Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, SwitzerlandInstitute of Diagnostic and Interventional Neuroradiology, Inselspital, Bern University Hospital, Bern, SwitzerlandDepartment of Chemistry and Applied Biosciences, Swiss Federal Institute of Technology, Zurich, SwitzerlandInstitute of Diagnostic and Interventional Neuroradiology, Inselspital, Bern University Hospital, Bern, SwitzerlandDepartment of Diagnostic and Interventional Neuroradiology, Alfried Krupp Hospital, Essen, GermanyLaboratory of Biomechanical Orthopedics, École Polytechnique Fédérale de Lausanne, Lausanne, SwitzerlandAn alternative intracranial aneurysm embolic agent is emerging in the form of hydrogels due to their ability to be injected in liquid phase and solidify in situ. Hydrogels have the ability to fill an aneurysm sac more completely compared to solid implants such as those used in coil embolization. Recently, the feasibility to implement photopolymerizable poly(ethylene glycol) dimethacrylate (PEGDMA) hydrogels in vitro has been demonstrated for aneurysm application. Nonetheless, the physical and mechanical properties of such hydrogels require further characterization to evaluate their long-term integrity and stability to avoid implant compaction and aneurysm recurrence over time. To that end, molecular weight and polymer content of the hydrogels were tuned to match the elastic modulus and compliance of aneurysmal tissue while minimizing the swelling volume and pressure. The hydrogel precursor was injected and photopolymerized in an in vitro aneurysm model, designed by casting polydimethylsiloxane (PDMS) around 3D printed water-soluble sacrificial molds. The hydrogels were then exposed to a fatigue test under physiological pulsatile flow, inducing a combination of circumferential and shear stresses. The hydrogels withstood 5.5 million cycles and no significant weight loss of the implant was observed nor did the polymerized hydrogel protrude or migrate into the parent artery. Slight surface erosion defects of 2–10 μm in depth were observed after loading compared to 2 μm maximum for non-loaded hydrogels. These results show that our fine-tuned photopolymerized hydrogel is expected to withstand the physiological conditions of an in vivo implant study.https://www.frontiersin.org/articles/10.3389/fbioe.2020.619858/fullpulsatile fluid flow-induced loadingintracranial aneurysmspolyethylene glycol dimethacrylatehydrogelsfatigueerosion
collection DOAJ
language English
format Article
sources DOAJ
author Oriane Poupart
Riccardo Conti
Andreas Schmocker
Andreas Schmocker
Andreas Schmocker
Lucio Pancaldi
Christophe Moser
Katja M. Nuss
Mahmut S. Sakar
Tomas Dobrocky
Hansjörg Grützmacher
Pascal J. Mosimann
Pascal J. Mosimann
Dominique P. Pioletti
spellingShingle Oriane Poupart
Riccardo Conti
Andreas Schmocker
Andreas Schmocker
Andreas Schmocker
Lucio Pancaldi
Christophe Moser
Katja M. Nuss
Mahmut S. Sakar
Tomas Dobrocky
Hansjörg Grützmacher
Pascal J. Mosimann
Pascal J. Mosimann
Dominique P. Pioletti
Pulsatile Flow-Induced Fatigue-Resistant Photopolymerizable Hydrogels for the Treatment of Intracranial Aneurysms
Frontiers in Bioengineering and Biotechnology
pulsatile fluid flow-induced loading
intracranial aneurysms
polyethylene glycol dimethacrylate
hydrogels
fatigue
erosion
author_facet Oriane Poupart
Riccardo Conti
Andreas Schmocker
Andreas Schmocker
Andreas Schmocker
Lucio Pancaldi
Christophe Moser
Katja M. Nuss
Mahmut S. Sakar
Tomas Dobrocky
Hansjörg Grützmacher
Pascal J. Mosimann
Pascal J. Mosimann
Dominique P. Pioletti
author_sort Oriane Poupart
title Pulsatile Flow-Induced Fatigue-Resistant Photopolymerizable Hydrogels for the Treatment of Intracranial Aneurysms
title_short Pulsatile Flow-Induced Fatigue-Resistant Photopolymerizable Hydrogels for the Treatment of Intracranial Aneurysms
title_full Pulsatile Flow-Induced Fatigue-Resistant Photopolymerizable Hydrogels for the Treatment of Intracranial Aneurysms
title_fullStr Pulsatile Flow-Induced Fatigue-Resistant Photopolymerizable Hydrogels for the Treatment of Intracranial Aneurysms
title_full_unstemmed Pulsatile Flow-Induced Fatigue-Resistant Photopolymerizable Hydrogels for the Treatment of Intracranial Aneurysms
title_sort pulsatile flow-induced fatigue-resistant photopolymerizable hydrogels for the treatment of intracranial aneurysms
publisher Frontiers Media S.A.
series Frontiers in Bioengineering and Biotechnology
issn 2296-4185
publishDate 2021-01-01
description An alternative intracranial aneurysm embolic agent is emerging in the form of hydrogels due to their ability to be injected in liquid phase and solidify in situ. Hydrogels have the ability to fill an aneurysm sac more completely compared to solid implants such as those used in coil embolization. Recently, the feasibility to implement photopolymerizable poly(ethylene glycol) dimethacrylate (PEGDMA) hydrogels in vitro has been demonstrated for aneurysm application. Nonetheless, the physical and mechanical properties of such hydrogels require further characterization to evaluate their long-term integrity and stability to avoid implant compaction and aneurysm recurrence over time. To that end, molecular weight and polymer content of the hydrogels were tuned to match the elastic modulus and compliance of aneurysmal tissue while minimizing the swelling volume and pressure. The hydrogel precursor was injected and photopolymerized in an in vitro aneurysm model, designed by casting polydimethylsiloxane (PDMS) around 3D printed water-soluble sacrificial molds. The hydrogels were then exposed to a fatigue test under physiological pulsatile flow, inducing a combination of circumferential and shear stresses. The hydrogels withstood 5.5 million cycles and no significant weight loss of the implant was observed nor did the polymerized hydrogel protrude or migrate into the parent artery. Slight surface erosion defects of 2–10 μm in depth were observed after loading compared to 2 μm maximum for non-loaded hydrogels. These results show that our fine-tuned photopolymerized hydrogel is expected to withstand the physiological conditions of an in vivo implant study.
topic pulsatile fluid flow-induced loading
intracranial aneurysms
polyethylene glycol dimethacrylate
hydrogels
fatigue
erosion
url https://www.frontiersin.org/articles/10.3389/fbioe.2020.619858/full
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