Modification of RSF1010-Based Broad-Host-Range Plasmids for Improved Conjugation and Cyanobacterial Bioprospecting

Summary: To facilitate the genetic engineering of diverse cyanobacterial strains, we have modified broad-host-range RSF1010-based plasmids to improve transmissibility, increase copy number, and facilitate cloning. RSF1010-based plasmids replicate in diverse bacterial strains but produce low amounts...

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Bibliographic Details
Main Authors: Bryan Bishé, Arnaud Taton, James W. Golden
Format: Article
Language:English
Published: Elsevier 2019-10-01
Series:iScience
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004219303396