GINGIVAL FIBROBLASTS AND NAK-1-ACTIVATED PRO-B LYMPHOCYTES APOPTOSIS

• Main Authors: Carmen Gentimir (Amititeloaie), Anda Cătălina Zaharia, Eugen Bitere, Dragoş Acatrinei, Ionuţ Cazan, Marcel Costuleanu, Otilia Boişteanu
• Format: Article
• Language: English
• Published: Romanian Society of Oral Rehabilitation 2020-12-01
• Series: Romanian Journal of Oral Rehabilitation
• Subjects: apoptosis; autophagy; gingival fibroblast; nak-1; pro-b lymphocyte
• Online Access: http://www.rjor.ro/gingival-fibroblasts-and-nak-1-activated-pro-b-lymphocytes-apoptosis/

Aim of the study We studied the effects of fibroblasts autophagy on the apoptosis of pro-B murine lymphocytes when were co-cultured. Material and methods Study of literature suggested that released autophagy-derived metabolites from fibroblasts might affect the apoptotic behavior of lymphocytes when there is a close interaction of these cells. The experimental plan of the actual studies was represented by induced effects of the presence of gingival fibroblasts in co-culture on NAK-1 receptors-activated pro-lymphocytes B apoptosis. Results We clearly showed that murine pro-B lymphocyte apoptosis induced by indol-3-carbinol could be significantly enhanced by the presence of gingival fibroblasts in co-culture. Furthermore, the addition of 3-methyl-adenine in the medium for 24 hours blocked the enhancing effects induced by fibroblasts on the apoptotic index of pro-B lymphocytes. Conclusions So, the enhancing apoptotic effects of fibroblasts on pro-B lymphocytes are related to released autophagy-derived metabolites. Their real nature remains to be established through future researches involving this complex system.