Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents
By coating surfaces with nano-crystalline diamond (NCD) particles, hydrophilicity can be altered via sidechain modifications without affecting surface texture. The present study aimed to assess the impact of NCD hydrophilicity on machined and rough SLA titanium discs on soft tissue integration, usin...
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doaj-719eb5c21feb4af4b4250c3cf971cbfa2020-11-24T22:20:05ZengMDPI AGNanomaterials2079-49912018-07-018752410.3390/nano8070524nano8070524Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in RodentsRobert Gerhard Stigler0Kathrin Becker1Michela Bruschi2Doris Steinmüller-Nethl3Robert Gassner4Department of Oral and Maxillofacial Surgery, Medical University Innsbruck, 6020 Innsbruck, AustriaDepartment of Orthodontics, Universitätsklinikum Düsseldorf, 40225 Düsseldorf, GermanyDepartment of Oral and Maxillofacial Surgery, Medical University Innsbruck, 6020 Innsbruck, AustriaDiaCoating GmbH, 6112 Wattens, AustriaDepartment of Oral and Maxillofacial Surgery, Medical University Innsbruck, 6020 Innsbruck, AustriaBy coating surfaces with nano-crystalline diamond (NCD) particles, hydrophilicity can be altered via sidechain modifications without affecting surface texture. The present study aimed to assess the impact of NCD hydrophilicity on machined and rough SLA titanium discs on soft tissue integration, using a rodent model simulating submerged healing. Four different titanium discs (machined titanium = M Titanium, NCD-coated hydrophilic machined titanium = M-O-NCD, sand blasted acid etched (SLA Titanium) titanium, and hydrophilic NCD-coated SLA titanium = SLA O-NCD) were inserted in subdermal pockets of 12 Wistar rats. After one and four weeks of healing, the animals were sacrificed. Biopsies were embedded in methyl methacrylate (MMA), and processed for histology. The number of cells located within a region of interest (ROI) of 10 µm around the discs were counted and compared statistically. Signs of inflammation were evaluated descriptively employing immunohistochemistry. At one week, M-O-NCD coated titanium discs showed significantly higher amounts of cells compared to M Titanium, SLA Titanium, and SLA-O-NCD (p < 0.001). At four weeks, significant higher cell counts were noted at SLA-O-NCD surfaces (p < 0.01). Immunohistochemistry revealed decreased inflammatory responses at hydrophilic surfaces. Within the limits of an animal study, M-O-NCD surfaces seem to stimulate cell proliferation in the initial healing phase, whereas SLA-O-NCD surfaces appeared advantageous afterwards.http://www.mdpi.com/2079-4991/8/7/524cell proliferationsoft-tissue healingchemical surface modificationsurface treatmenthydrophilicitiynano-crystalline diamond coating |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Robert Gerhard Stigler Kathrin Becker Michela Bruschi Doris Steinmüller-Nethl Robert Gassner |
spellingShingle |
Robert Gerhard Stigler Kathrin Becker Michela Bruschi Doris Steinmüller-Nethl Robert Gassner Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents Nanomaterials cell proliferation soft-tissue healing chemical surface modification surface treatment hydrophilicitiy nano-crystalline diamond coating |
author_facet |
Robert Gerhard Stigler Kathrin Becker Michela Bruschi Doris Steinmüller-Nethl Robert Gassner |
author_sort |
Robert Gerhard Stigler |
title |
Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents |
title_short |
Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents |
title_full |
Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents |
title_fullStr |
Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents |
title_full_unstemmed |
Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents |
title_sort |
impact of nano-crystalline diamond enhanced hydrophilicity on cell proliferation on machined and sla titanium surfaces: an in-vivo study in rodents |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2018-07-01 |
description |
By coating surfaces with nano-crystalline diamond (NCD) particles, hydrophilicity can be altered via sidechain modifications without affecting surface texture. The present study aimed to assess the impact of NCD hydrophilicity on machined and rough SLA titanium discs on soft tissue integration, using a rodent model simulating submerged healing. Four different titanium discs (machined titanium = M Titanium, NCD-coated hydrophilic machined titanium = M-O-NCD, sand blasted acid etched (SLA Titanium) titanium, and hydrophilic NCD-coated SLA titanium = SLA O-NCD) were inserted in subdermal pockets of 12 Wistar rats. After one and four weeks of healing, the animals were sacrificed. Biopsies were embedded in methyl methacrylate (MMA), and processed for histology. The number of cells located within a region of interest (ROI) of 10 µm around the discs were counted and compared statistically. Signs of inflammation were evaluated descriptively employing immunohistochemistry. At one week, M-O-NCD coated titanium discs showed significantly higher amounts of cells compared to M Titanium, SLA Titanium, and SLA-O-NCD (p < 0.001). At four weeks, significant higher cell counts were noted at SLA-O-NCD surfaces (p < 0.01). Immunohistochemistry revealed decreased inflammatory responses at hydrophilic surfaces. Within the limits of an animal study, M-O-NCD surfaces seem to stimulate cell proliferation in the initial healing phase, whereas SLA-O-NCD surfaces appeared advantageous afterwards. |
topic |
cell proliferation soft-tissue healing chemical surface modification surface treatment hydrophilicitiy nano-crystalline diamond coating |
url |
http://www.mdpi.com/2079-4991/8/7/524 |
work_keys_str_mv |
AT robertgerhardstigler impactofnanocrystallinediamondenhancedhydrophilicityoncellproliferationonmachinedandslatitaniumsurfacesaninvivostudyinrodents AT kathrinbecker impactofnanocrystallinediamondenhancedhydrophilicityoncellproliferationonmachinedandslatitaniumsurfacesaninvivostudyinrodents AT michelabruschi impactofnanocrystallinediamondenhancedhydrophilicityoncellproliferationonmachinedandslatitaniumsurfacesaninvivostudyinrodents AT dorissteinmullernethl impactofnanocrystallinediamondenhancedhydrophilicityoncellproliferationonmachinedandslatitaniumsurfacesaninvivostudyinrodents AT robertgassner impactofnanocrystallinediamondenhancedhydrophilicityoncellproliferationonmachinedandslatitaniumsurfacesaninvivostudyinrodents |
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1725776992450641920 |