Influence of luteolin on the apoptosis of esophageal cancer Eca109 cells and its mechanism of action

The present study was conducted to verify the influence of luteolin on apoptosis of Eca109 cells and to further investigate the possible mechanisms underlying its effect on apoptosis. The cells were exposed to different concentrations of luteolin (0, 40, 80, 120, 160, 200, 240 μM) for 24, 48, and 72...

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Main Authors: Shaokang Wang, Lingmeng Fu, Yi Wu, Hongmei Xiao, Jing Wang, Guiju Sun
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2019-06-01
Series:Food Science and Human Wellness
Online Access:http://www.sciencedirect.com/science/article/pii/S2213453019300424
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spelling doaj-718dfc805f714b0da1a5042b70b2acf32021-02-02T01:56:41ZengKeAi Communications Co., Ltd.Food Science and Human Wellness2213-45302019-06-0182189194Influence of luteolin on the apoptosis of esophageal cancer Eca109 cells and its mechanism of actionShaokang Wang0Lingmeng Fu1Yi Wu2Hongmei Xiao3Jing Wang4Guiju Sun5Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, PR ChinaKey Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, PR ChinaKey Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, PR ChinaKey Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, PR ChinaKey Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, PR ChinaCorresponding author at: Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, No. 87 Dingjiaqiao, Gulou District, Nanjing 210009, PR China.; Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, PR ChinaThe present study was conducted to verify the influence of luteolin on apoptosis of Eca109 cells and to further investigate the possible mechanisms underlying its effect on apoptosis. The cells were exposed to different concentrations of luteolin (0, 40, 80, 120, 160, 200, 240 μM) for 24, 48, and 72 h respectively. The influence of luteolin on proliferation of Eca109 cells was detected using MTT assay. Eca109 cells were then treated with luteolin (0, 40, 160, 240 μM) for 24 h. The effect of luteolin on cell cycle progression and apoptosis was assayed by using flow cytometry (FCM). Expression of caspase9 and caspase3 mRNA and protein was analyzed by real-time PCR and Western blot respectively. The results showed that luteolin could inhibit the proliferation of Eca109 cells at all concentrations in a time-dependent manner and the relative inhibition rate showed an inverted U-shaped association with the concentration of luteolin. Further, the cell cycle was arrested in the S phase following treatment with luteolin. Apoptosis analysis indicated that luteolin could induce the apoptosis of Eca109 cells across the three concentration groups, which exhibited a trend of first promotional and then inhibitory with the increases in luteolin concentration. The effect of luteolin on the mRNA and protein expression of caspase 9 and caspase3 first manifested as promotion, then inhibition. Therefore, luteolin may serve a role in promoting cell apoptosis by inducing Eca109 cell apoptosis that involves the expression of caspase3, caspase9 mRNA and protein. This study provides theoretical basis for further study and clinical application of luteolin. The specific mechanism has not yet been clarified and the other activation pathways inducing apoptosis need to be further studied. Keywords: Esophageal cancer, Luteolin, Apoptosishttp://www.sciencedirect.com/science/article/pii/S2213453019300424
collection DOAJ
language English
format Article
sources DOAJ
author Shaokang Wang
Lingmeng Fu
Yi Wu
Hongmei Xiao
Jing Wang
Guiju Sun
spellingShingle Shaokang Wang
Lingmeng Fu
Yi Wu
Hongmei Xiao
Jing Wang
Guiju Sun
Influence of luteolin on the apoptosis of esophageal cancer Eca109 cells and its mechanism of action
Food Science and Human Wellness
author_facet Shaokang Wang
Lingmeng Fu
Yi Wu
Hongmei Xiao
Jing Wang
Guiju Sun
author_sort Shaokang Wang
title Influence of luteolin on the apoptosis of esophageal cancer Eca109 cells and its mechanism of action
title_short Influence of luteolin on the apoptosis of esophageal cancer Eca109 cells and its mechanism of action
title_full Influence of luteolin on the apoptosis of esophageal cancer Eca109 cells and its mechanism of action
title_fullStr Influence of luteolin on the apoptosis of esophageal cancer Eca109 cells and its mechanism of action
title_full_unstemmed Influence of luteolin on the apoptosis of esophageal cancer Eca109 cells and its mechanism of action
title_sort influence of luteolin on the apoptosis of esophageal cancer eca109 cells and its mechanism of action
publisher KeAi Communications Co., Ltd.
series Food Science and Human Wellness
issn 2213-4530
publishDate 2019-06-01
description The present study was conducted to verify the influence of luteolin on apoptosis of Eca109 cells and to further investigate the possible mechanisms underlying its effect on apoptosis. The cells were exposed to different concentrations of luteolin (0, 40, 80, 120, 160, 200, 240 μM) for 24, 48, and 72 h respectively. The influence of luteolin on proliferation of Eca109 cells was detected using MTT assay. Eca109 cells were then treated with luteolin (0, 40, 160, 240 μM) for 24 h. The effect of luteolin on cell cycle progression and apoptosis was assayed by using flow cytometry (FCM). Expression of caspase9 and caspase3 mRNA and protein was analyzed by real-time PCR and Western blot respectively. The results showed that luteolin could inhibit the proliferation of Eca109 cells at all concentrations in a time-dependent manner and the relative inhibition rate showed an inverted U-shaped association with the concentration of luteolin. Further, the cell cycle was arrested in the S phase following treatment with luteolin. Apoptosis analysis indicated that luteolin could induce the apoptosis of Eca109 cells across the three concentration groups, which exhibited a trend of first promotional and then inhibitory with the increases in luteolin concentration. The effect of luteolin on the mRNA and protein expression of caspase 9 and caspase3 first manifested as promotion, then inhibition. Therefore, luteolin may serve a role in promoting cell apoptosis by inducing Eca109 cell apoptosis that involves the expression of caspase3, caspase9 mRNA and protein. This study provides theoretical basis for further study and clinical application of luteolin. The specific mechanism has not yet been clarified and the other activation pathways inducing apoptosis need to be further studied. Keywords: Esophageal cancer, Luteolin, Apoptosis
url http://www.sciencedirect.com/science/article/pii/S2213453019300424
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