Ceftriaxone-Induced Immune Hemolytic Anemia: In Vitro Reversal with Peptide Inhibitor of Complement C1 (PIC1)

Ceftriaxone-Induced Immune Hemolytic Anemia: In Vitro Reversal with Peptide Inhibitor of Complement C1 (PIC1)

We report a case of ceftriaxone-induced immune hemolytic anemia in a 10-year-old with chronic active Epstein–Barr virus disease and hemophagocytic lymphohistiocytosis. After chemotherapy, she became febrile and received ceftriaxone. She rapidly developed respiratory failure and anemia. Her direct an...

Full description

Bibliographic Details
Main Authors: Kenji M. Cunnion, Lisa M. Feagin, Michael F. Chicella, Cortney L. Kaszowski, Pamela S. Hair, Jessica Price, William C. Owen
Format: Article
Language:English
Published: Hindawi Limited 2019-01-01
Series:Case Reports in Hematology
Online Access:http://dx.doi.org/10.1155/2019/4105653
id doaj-71712697d0ea4195ad3373573e59fce5
record_format Article
spelling doaj-71712697d0ea4195ad3373573e59fce52020-11-24T22:13:44ZengHindawi LimitedCase Reports in Hematology2090-65602090-65792019-01-01201910.1155/2019/41056534105653Ceftriaxone-Induced Immune Hemolytic Anemia: In Vitro Reversal with Peptide Inhibitor of Complement C1 (PIC1)Kenji M. Cunnion0Lisa M. Feagin1Michael F. Chicella2Cortney L. Kaszowski3Pamela S. Hair4Jessica Price5William C. Owen6Department of Pediatrics, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, VA 23507, USADepartment of Pharmacy, Children’s Hospital of the King’s Daughters, 601 Children’s Lane, Norfolk, VA 23507, USADepartment of Pediatrics, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, VA 23507, USADepartment of Pediatrics, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, VA 23507, USADepartment of Pediatrics, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, VA 23507, USADepartment of Pediatrics, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, VA 23507, USADepartment of Pediatrics, Eastern Virginia Medical School, 700 West Olney Road, Norfolk, VA 23507, USAWe report a case of ceftriaxone-induced immune hemolytic anemia in a 10-year-old with chronic active Epstein–Barr virus disease and hemophagocytic lymphohistiocytosis. After chemotherapy, she became febrile and received ceftriaxone. She rapidly developed respiratory failure and anemia. Her direct antiglobulin test was positive for IgG and C3. To confirm this was ceftriaxone-induced complement-mediated hemolysis, we adapted the complement hemolysis using human erythrocytes (CHUHE) assay by adding exogenous ceftriaxone to the patient’s serum which enhanced lysis of her erythrocytes. We confirmed that ceftriaxone initiated a classical complement pathway-mediated hemolysis by in vitro reversal with peptide inhibitor of complement C1 (PIC1).http://dx.doi.org/10.1155/2019/4105653
collection DOAJ
language English
format Article
sources DOAJ
author Kenji M. Cunnion
Lisa M. Feagin
Michael F. Chicella
Cortney L. Kaszowski
Pamela S. Hair
Jessica Price
William C. Owen
spellingShingle Kenji M. Cunnion
Lisa M. Feagin
Michael F. Chicella
Cortney L. Kaszowski
Pamela S. Hair
Jessica Price
William C. Owen
Ceftriaxone-Induced Immune Hemolytic Anemia: In Vitro Reversal with Peptide Inhibitor of Complement C1 (PIC1)
Case Reports in Hematology
author_facet Kenji M. Cunnion
Lisa M. Feagin
Michael F. Chicella
Cortney L. Kaszowski
Pamela S. Hair
Jessica Price
William C. Owen
author_sort Kenji M. Cunnion
title Ceftriaxone-Induced Immune Hemolytic Anemia: In Vitro Reversal with Peptide Inhibitor of Complement C1 (PIC1)
title_short Ceftriaxone-Induced Immune Hemolytic Anemia: In Vitro Reversal with Peptide Inhibitor of Complement C1 (PIC1)
title_full Ceftriaxone-Induced Immune Hemolytic Anemia: In Vitro Reversal with Peptide Inhibitor of Complement C1 (PIC1)
title_fullStr Ceftriaxone-Induced Immune Hemolytic Anemia: In Vitro Reversal with Peptide Inhibitor of Complement C1 (PIC1)
title_full_unstemmed Ceftriaxone-Induced Immune Hemolytic Anemia: In Vitro Reversal with Peptide Inhibitor of Complement C1 (PIC1)
title_sort ceftriaxone-induced immune hemolytic anemia: in vitro reversal with peptide inhibitor of complement c1 (pic1)
publisher Hindawi Limited
series Case Reports in Hematology
issn 2090-6560
2090-6579
publishDate 2019-01-01
description We report a case of ceftriaxone-induced immune hemolytic anemia in a 10-year-old with chronic active Epstein–Barr virus disease and hemophagocytic lymphohistiocytosis. After chemotherapy, she became febrile and received ceftriaxone. She rapidly developed respiratory failure and anemia. Her direct antiglobulin test was positive for IgG and C3. To confirm this was ceftriaxone-induced complement-mediated hemolysis, we adapted the complement hemolysis using human erythrocytes (CHUHE) assay by adding exogenous ceftriaxone to the patient’s serum which enhanced lysis of her erythrocytes. We confirmed that ceftriaxone initiated a classical complement pathway-mediated hemolysis by in vitro reversal with peptide inhibitor of complement C1 (PIC1).
url http://dx.doi.org/10.1155/2019/4105653
work_keys_str_mv AT kenjimcunnion ceftriaxoneinducedimmunehemolyticanemiainvitroreversalwithpeptideinhibitorofcomplementc1pic1
AT lisamfeagin ceftriaxoneinducedimmunehemolyticanemiainvitroreversalwithpeptideinhibitorofcomplementc1pic1
AT michaelfchicella ceftriaxoneinducedimmunehemolyticanemiainvitroreversalwithpeptideinhibitorofcomplementc1pic1
AT cortneylkaszowski ceftriaxoneinducedimmunehemolyticanemiainvitroreversalwithpeptideinhibitorofcomplementc1pic1
AT pamelashair ceftriaxoneinducedimmunehemolyticanemiainvitroreversalwithpeptideinhibitorofcomplementc1pic1
AT jessicaprice ceftriaxoneinducedimmunehemolyticanemiainvitroreversalwithpeptideinhibitorofcomplementc1pic1
AT williamcowen ceftriaxoneinducedimmunehemolyticanemiainvitroreversalwithpeptideinhibitorofcomplementc1pic1
_version_ 1725799984237903872

Similar Items