Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa

The heat conduction microcalorimeter can be used to evaluate the metabolic rates of the sperm cell. Two ejaculates of four stallions were cooled to +5ºC and checked for sperm motility (bright field microscopy), viability (eosin 3%), functional membrane integrity (hyposmotic swelling test), and heat...

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Bibliographic Details
Main Authors: André Belico de Vasconcelos, Patrícia Castanheira de Souza, Fabiana Cristina Varago, Monique de Albuquerque Lagares, Marcelo Matos Santoro
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2009-10-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500010