Pre-column dervatization of amino acids from nigella sativa L seed hydrolysates by reversed phase HPLC
A rapid and sensitive method for analysis of amino acid hydrolysates of nigella sativa L seed has been developed using O-phthaldialehyde(OPA ) as a pre-column derivatizing agent. OPA reagents in the presence of mercaptoethanol react rapidly with primary amino acids ( less than 60 sec.) to form isin...
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| Format: | Article |
| Language: | Arabic |
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College of Science for Women, University of Baghdad
2008-03-01
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| Series: | Baghdad Science Journal |
| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/874 |