Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer

Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer

Resistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally encoded mutations or the recently discovered plasmid-encoded mcr genes that have further complicated the landscape of colistin...

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Main Authors: Laurent Dortet, Rémy A. Bonnin, Simon Le Hello, Laetitia Fabre, Richard Bonnet, Markus Kostrzewa, Alain Filloux, Gerald Larrouy-Maumus
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-06-01
Series:Frontiers in Microbiology
Subjects:
MALDI mass spectrometry
lipid A
colistin
Salmonella enterica
diagno stic
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.01141/full
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spelling doaj-7154d72b6cf24d29a529a1ae09db57da2020-11-25T03:00:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-06-011110.3389/fmicb.2020.01141519570Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass SpectrometerLaurent Dortet0Laurent Dortet1Laurent Dortet2Laurent Dortet3Rémy A. Bonnin4Rémy A. Bonnin5Rémy A. Bonnin6Simon Le Hello7Laetitia Fabre8Richard Bonnet9Richard Bonnet10Markus Kostrzewa11Alain Filloux12Gerald Larrouy-Maumus13MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, United KingdomDepartment of Bacteriology-Hygiene, Bicêtre Hospital, Assistance Publique – Hôpitaux de Paris, Le Kremlin-Bicêtre, FranceEA7361 “Structure, Dynamic, Function and Expression of Broad Spectrum ß-Lactamases”, Paris-Sud University, LabEx Lermit, Faculty of Medecine, Le Kremlin-Bicêtre, FranceFrench National Reference Centre for Antibiotic Resistance, Le Kremlin-Bicêtre, FranceDepartment of Bacteriology-Hygiene, Bicêtre Hospital, Assistance Publique – Hôpitaux de Paris, Le Kremlin-Bicêtre, FranceEA7361 “Structure, Dynamic, Function and Expression of Broad Spectrum ß-Lactamases”, Paris-Sud University, LabEx Lermit, Faculty of Medecine, Le Kremlin-Bicêtre, FranceFrench National Reference Centre for Antibiotic Resistance, Le Kremlin-Bicêtre, FranceInstitut Pasteur, French National Reference Centre for E. coli, Shigella and Salmonella, Paris, FranceInstitut Pasteur, French National Reference Centre for E. coli, Shigella and Salmonella, Paris, FranceFrench National Reference Centre for Antibiotic Resistance, Le Kremlin-Bicêtre, FranceDepartment of Bacteriology, University Hospital of Clermont-Ferrand, Clermont-Ferrand, FranceBruker Daltonik GmbH, Bremen, GermanyMRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, United KingdomMRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, United KingdomResistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally encoded mutations or the recently discovered plasmid-encoded mcr genes that have further complicated the landscape of colistin resistance. Currently, minimal inhibitory concentration (MIC) determination by broth microdilution, the gold standard for the detection of polymyxin resistance, is time consuming (24 h) and challenging to perform in clinical and veterinary laboratories. Here we present the use of the MALDIxin to detect colistin resistant Salmonella enterica using the MALDxin test on the routine matrix-assisted laser desorption ionization (MALDI) Biotyper Sirius system.https://www.frontiersin.org/article/10.3389/fmicb.2020.01141/fullMALDI mass spectrometrylipid AcolistinSalmonella entericadiagno stic
collection DOAJ
language English
format Article
sources DOAJ
author Laurent Dortet
Laurent Dortet
Laurent Dortet
Laurent Dortet
Rémy A. Bonnin
Rémy A. Bonnin
Rémy A. Bonnin
Simon Le Hello
Laetitia Fabre
Richard Bonnet
Richard Bonnet
Markus Kostrzewa
Alain Filloux
Gerald Larrouy-Maumus
spellingShingle Laurent Dortet
Laurent Dortet
Laurent Dortet
Laurent Dortet
Rémy A. Bonnin
Rémy A. Bonnin
Rémy A. Bonnin
Simon Le Hello
Laetitia Fabre
Richard Bonnet
Richard Bonnet
Markus Kostrzewa
Alain Filloux
Gerald Larrouy-Maumus
Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
Frontiers in Microbiology
MALDI mass spectrometry
lipid A
colistin
Salmonella enterica
diagno stic
author_facet Laurent Dortet
Laurent Dortet
Laurent Dortet
Laurent Dortet
Rémy A. Bonnin
Rémy A. Bonnin
Rémy A. Bonnin
Simon Le Hello
Laetitia Fabre
Richard Bonnet
Richard Bonnet
Markus Kostrzewa
Alain Filloux
Gerald Larrouy-Maumus
author_sort Laurent Dortet
title Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
title_short Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
title_full Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
title_fullStr Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
title_full_unstemmed Detection of Colistin Resistance in Salmonella enterica Using MALDIxin Test on the Routine MALDI Biotyper Sirius Mass Spectrometer
title_sort detection of colistin resistance in salmonella enterica using maldixin test on the routine maldi biotyper sirius mass spectrometer
publisher Frontiers Media S.A.
series Frontiers in Microbiology
issn 1664-302X
publishDate 2020-06-01
description Resistance to polymyxins in most Gram-negative bacteria arises from chemical modifications to the lipid A portion of their lipopolysaccharide (LPS) mediated by chromosomally encoded mutations or the recently discovered plasmid-encoded mcr genes that have further complicated the landscape of colistin resistance. Currently, minimal inhibitory concentration (MIC) determination by broth microdilution, the gold standard for the detection of polymyxin resistance, is time consuming (24 h) and challenging to perform in clinical and veterinary laboratories. Here we present the use of the MALDIxin to detect colistin resistant Salmonella enterica using the MALDxin test on the routine matrix-assisted laser desorption ionization (MALDI) Biotyper Sirius system.
topic MALDI mass spectrometry
lipid A
colistin
Salmonella enterica
diagno stic
url https://www.frontiersin.org/article/10.3389/fmicb.2020.01141/full
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