Production of α-Amylase from Penicillium chrysogenum under Solid-State Fermentation by Using Some Agricultural By-Products
Solid-state fermentation (SSF) was carried out using corncob leaf (CL), rye straw (RS), wheat straw (WS) and wheat bran (WB) as substrates for α-amylase production by a fungal culture of Penicillium chrysogenum. The effects of moisture level, particle size and inoculum concentration on enzyme synthe...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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University of Zagreb
2007-01-01
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| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | http://hrcak.srce.hr/file/37784 |
| Summary: | Solid-state fermentation (SSF) was carried out using corncob leaf (CL), rye straw (RS), wheat straw (WS) and wheat bran (WB) as substrates for α-amylase production by a fungal culture of Penicillium chrysogenum. The effects of moisture level, particle size and inoculum concentration on enzyme synthesis from P. chrysogenum were investigated. Optimal moisture levels of substrates were 75, 65, 65 and 55 % for CL, WS, WB and RS substrates, respectively. Optimal particle size and inoculum concentration for the production of α-amylase were: >1 mm, 20 %; >1 mm, 20 %; 1 mm, 20 % and >1 mm, 30 % for CL, WS, WB and RS, respectively. WB showed the highest enzyme production with 160 U/mL under optimum conditions. The other enzyme activities were 28, 49 and 45 U/mL using CL, RS and WS, respectively. |
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| ISSN: | 1330-9862 1334-2606 |