The Oligomeric State of the Plasma Membrane H+-ATPase from Kluyveromyces lactis

The Oligomeric State of the Plasma Membrane H+-ATPase from Kluyveromyces lactis

The plasma membrane H+-ATPase was purified from the yeast K. lactis. The oligomeric state of the H+-ATPase is not known. Size exclusion chromatography displayed two macromolecular assembly states (MASs) of different sizes for the solubilized enzyme. Blue native electrophoresis (BN-PAGE) showed the H...

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Bibliographic Details
Main Authors: Yadira G. Ruiz-Granados, Valentín De La Cruz-Torres, José G. Sampedro
Format: Article
Language:English
Published: MDPI AG 2019-03-01
Series:Molecules
Subjects:
H+-ATPase
hexamer
macromolecular assembly
enzyme kinetics
fluorescence
binding site affinity
Online Access:http://www.mdpi.com/1420-3049/24/5/958

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