TRIAMF: A New Method for Delivery of Cas9 Ribonucleoprotein Complex to Human Hematopoietic Stem Cells

TRIAMF: A New Method for Delivery of Cas9 Ribonucleoprotein Complex to Human Hematopoietic Stem Cells

Abstract CRISPR/Cas9 mediated gene editing of patient-derived hematopoietic stem and progenitor cells (HSPCs) ex vivo followed by autologous transplantation of the edited HSPCs back to the patient can provide a potential cure for monogenic blood disorders such as β-hemoglobinopathies. One challenge...

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Bibliographic Details
Main Authors: Jonathan Yen, Michael Fiorino, Yi Liu, Steve Paula, Scott Clarkson, Lisa Quinn, William R. Tschantz, Heath Klock, Ning Guo, Carsten Russ, Vionnie W. C. Yu, Craig Mickanin, Susan C. Stevenson, Cameron Lee, Yi Yang
Format: Article
Language:English
Published: Nature Publishing Group 2018-11-01
Series:Scientific Reports
Subjects:
Hematopoietic Stem And Progenitor Cells (HSPCs)
Purified Cas9 Protein
HSPC Expansion
Neon Electroporation
Syringe Connector
Online Access:https://doi.org/10.1038/s41598-018-34601-6

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