MicroRNA-205-5p inhibits skin cancer cell proliferation and increase drug sensitivity by targeting TNFAIP8
Abstract Tumor necrosis factor-α-induced protein 8 (TNFAIP8) is a member of the TIPE/TNFAIP8 family which regulates tumor growth and survival. Our goal is to delineate the detailed oncogenic role of TNFAIP8 in skin cancer development and progression. Here we demonstrated that higher expression of TN...
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doaj-710c00f389104119aaf99dc155064fa12021-03-11T12:20:34ZengNature Publishing GroupScientific Reports2045-23222021-03-0111111410.1038/s41598-021-85097-6MicroRNA-205-5p inhibits skin cancer cell proliferation and increase drug sensitivity by targeting TNFAIP8Xinhong Ge0Suryakant Niture1Minghui Lin2Patrice Cagle3P. Andy Li4Deepak Kumar5Department of Dermatology, General Hospital of Ningxia Medical UniversityJulius L. Chambers Biomedical Biotechnology Research Institute (BBRI), North Carolina Central UniversityDepartment of Respiratory Diseases, The Forth People’s Hospital of Ningxia Hui Autonomous RegionJulius L. Chambers Biomedical Biotechnology Research Institute (BBRI), North Carolina Central UniversityDepartment of Pharmaceutical Sciences, Bio-Manufacturing Research Institute and Technology Enterprise (BRITE), College of Health and Sciences, North Carolina Central UniversityJulius L. Chambers Biomedical Biotechnology Research Institute (BBRI), North Carolina Central UniversityAbstract Tumor necrosis factor-α-induced protein 8 (TNFAIP8) is a member of the TIPE/TNFAIP8 family which regulates tumor growth and survival. Our goal is to delineate the detailed oncogenic role of TNFAIP8 in skin cancer development and progression. Here we demonstrated that higher expression of TNFAIP8 is associated with basal cell carcinoma (BCC), squamous cell carcinoma (SCC), and melanoma development in patient tissues. Induction of TNFAIP8 expression by TNFα or by ectopic expression of TNFAIP8 in SCC or melanoma cell lines resulted in increased cell growth/proliferation. Conversely, silencing of TNFAIP8 decreased cell survival/cell migration in skin cancer cells. We also showed that miR-205-5p targets the 3′UTR of TNFAIP8 and inhibits TNFAIP8 expression. Moreover, miR-205-5p downregulates TNFAIP8 mediated cellular autophagy, increased sensitivity towards the B-RAFV600E mutant kinase inhibitor vemurafenib, and induced cell apoptosis in melanoma cells. Collectively our data indicate that miR-205-5p acts as a tumor suppressor in skin cancer by targeting TNFAIP8.https://doi.org/10.1038/s41598-021-85097-6 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xinhong Ge Suryakant Niture Minghui Lin Patrice Cagle P. Andy Li Deepak Kumar |
spellingShingle |
Xinhong Ge Suryakant Niture Minghui Lin Patrice Cagle P. Andy Li Deepak Kumar MicroRNA-205-5p inhibits skin cancer cell proliferation and increase drug sensitivity by targeting TNFAIP8 Scientific Reports |
author_facet |
Xinhong Ge Suryakant Niture Minghui Lin Patrice Cagle P. Andy Li Deepak Kumar |
author_sort |
Xinhong Ge |
title |
MicroRNA-205-5p inhibits skin cancer cell proliferation and increase drug sensitivity by targeting TNFAIP8 |
title_short |
MicroRNA-205-5p inhibits skin cancer cell proliferation and increase drug sensitivity by targeting TNFAIP8 |
title_full |
MicroRNA-205-5p inhibits skin cancer cell proliferation and increase drug sensitivity by targeting TNFAIP8 |
title_fullStr |
MicroRNA-205-5p inhibits skin cancer cell proliferation and increase drug sensitivity by targeting TNFAIP8 |
title_full_unstemmed |
MicroRNA-205-5p inhibits skin cancer cell proliferation and increase drug sensitivity by targeting TNFAIP8 |
title_sort |
microrna-205-5p inhibits skin cancer cell proliferation and increase drug sensitivity by targeting tnfaip8 |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-03-01 |
description |
Abstract Tumor necrosis factor-α-induced protein 8 (TNFAIP8) is a member of the TIPE/TNFAIP8 family which regulates tumor growth and survival. Our goal is to delineate the detailed oncogenic role of TNFAIP8 in skin cancer development and progression. Here we demonstrated that higher expression of TNFAIP8 is associated with basal cell carcinoma (BCC), squamous cell carcinoma (SCC), and melanoma development in patient tissues. Induction of TNFAIP8 expression by TNFα or by ectopic expression of TNFAIP8 in SCC or melanoma cell lines resulted in increased cell growth/proliferation. Conversely, silencing of TNFAIP8 decreased cell survival/cell migration in skin cancer cells. We also showed that miR-205-5p targets the 3′UTR of TNFAIP8 and inhibits TNFAIP8 expression. Moreover, miR-205-5p downregulates TNFAIP8 mediated cellular autophagy, increased sensitivity towards the B-RAFV600E mutant kinase inhibitor vemurafenib, and induced cell apoptosis in melanoma cells. Collectively our data indicate that miR-205-5p acts as a tumor suppressor in skin cancer by targeting TNFAIP8. |
url |
https://doi.org/10.1038/s41598-021-85097-6 |
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