Activity of the mitochondrial isoenzymes of endogenous aldehydes catabolism under the conditions of acetaminophen-induced hepatitis

• Main Authors: O. M. Voloshchuk, G. P. Kopylchuk, Y. I. Mishyna
• Format: Article
• Language: English
• Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2018-02-01
• Series: Ukrainian Biochemical Journal
• Subjects: aldehyde dehydrogenase; aldehyde reductase; alimentary protein deficiency; hepatotoxicity; mitochondria; protein carbonyl derivatives; TBA reactive substances
• Online Access: http://ukrbiochemjournal.org/2018/02/activity-of-the-mitochondrial-isoenzymes-of-endogenous-aldehydes-catabolism-under-the-conditions-of-acetaminophen-induced-hepatitis.html

The research deals with the determination of the activity of aldehyde dehydrogenase (EC 1.2.1.3), aldehyde reductase (EC 1.1.1.21) as well as the content of TBA-active products and protein carbonyl derivates in the rat liver cytosolic fraction under the conditions of acetaminophen-induced hepatitis and protein deficiency. The most pronounced decrease in the activity of enzymes utilizing endogenous aldehydes is observed in the liver cytosolic fraction of animals with toxic liver injury maintained under the conditions of alimentary protein deficiency. Meanwhile, the accumulation of TBA-active products and protein carbonyl-derivates in the liver cytosolic fraction of animals of this experimental group was established. The accumulation of aldehyde products of lipid and protein oxidative damage on the background of the reduction in the activity of enzymes providing aldehyde catabolism may be considered as a possible mechanism underlying hepatocyte dysfunction under the conditions of toxic damage in protein-deficient animals.