Characterization of non-heme iron aliphatic halogenase WelO5* from Hapalosiphon welwitschii IC-52-3: Identification of a minimal protein sequence motif that confers enzymatic chlorination specificity in the biosynthesis of welwitindolelinones

Characterization of non-heme iron aliphatic halogenase WelO5* from Hapalosiphon welwitschii IC-52-3: Identification of a minimal protein sequence motif that confers enzymatic chlorination specificity in the biosynthesis of welwitindolelinones

The in vitro biochemical characterization revealed that iron/2-oxoglutarate (Fe/2OG)-dependent aliphatic halogenase WelO5* in Hapalosiphon welwitschii IC-52-3 has an enhanced substrate specificity towards 12-epi-hapalindole C (1) in comparison to WelO5 in H. welwitschii UTEX B1830. This allowed us t...

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Bibliographic Details
Main Authors: Qin Zhu, Xinyu Liu
Format: Article
Language:English
Published: Beilstein-Institut 2017-06-01
Series:Beilstein Journal of Organic Chemistry
Subjects:
alkaloid biogenesis
biosynthetic divergency
C–H activation
halogenase
non-heme iron enzyme
Online Access:https://doi.org/10.3762/bjoc.13.115

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