Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common Carp <i>Cyprinus carpio</i>
The purpose of the current study was to analyze phenotypic and functional characteristics of common carp (<i>Cyprinus carpio</i>) spermatozoa during in vitro aging and to investigate whether global DNA methylation is affected by sperm aging. Milt was collected from five individual males,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/11/5925 |
| id |
doaj-70ae96d74e634f7e95007e53aeb1cf8d |
|---|---|
| record_format |
Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Yu Cheng Pavlina Vechtova Zoltan Fussy Jan Sterba Zuzana Linhartová Marek Rodina Vladimíra Tučková David Gela Azin Mohagheghi Samarin Ievgen Lebeda Miaomiao Xin Songpei Zhang Deepali Rahi Otomar Linhart |
| spellingShingle |
Yu Cheng Pavlina Vechtova Zoltan Fussy Jan Sterba Zuzana Linhartová Marek Rodina Vladimíra Tučková David Gela Azin Mohagheghi Samarin Ievgen Lebeda Miaomiao Xin Songpei Zhang Deepali Rahi Otomar Linhart Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common Carp <i>Cyprinus carpio</i> International Journal of Molecular Sciences fish common carp sperm aging epigenetics DNA methylation sperm quality |
| author_facet |
Yu Cheng Pavlina Vechtova Zoltan Fussy Jan Sterba Zuzana Linhartová Marek Rodina Vladimíra Tučková David Gela Azin Mohagheghi Samarin Ievgen Lebeda Miaomiao Xin Songpei Zhang Deepali Rahi Otomar Linhart |
| author_sort |
Yu Cheng |
| title |
Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common Carp <i>Cyprinus carpio</i> |
| title_short |
Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common Carp <i>Cyprinus carpio</i> |
| title_full |
Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common Carp <i>Cyprinus carpio</i> |
| title_fullStr |
Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common Carp <i>Cyprinus carpio</i> |
| title_full_unstemmed |
Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common Carp <i>Cyprinus carpio</i> |
| title_sort |
changes in phenotypes and dna methylation of in vitro aging sperm in common carp <i>cyprinus carpio</i> |
| publisher |
MDPI AG |
| series |
International Journal of Molecular Sciences |
| issn |
1661-6596 1422-0067 |
| publishDate |
2021-05-01 |
| description |
The purpose of the current study was to analyze phenotypic and functional characteristics of common carp (<i>Cyprinus carpio</i>) spermatozoa during in vitro aging and to investigate whether global DNA methylation is affected by sperm aging. Milt was collected from five individual males, stored in vitro on ice in a refrigerator for up to 96 h post stripping (HPS) and used to fertilize eggs with intervals of 1, 24 and 96 h. Computer-assisted sperm analysis and a S3e Cell Sorter was employed to determine the spermatozoa phenotypic characteristics (motility, velocity, concentration and viability). In addition, pH and osmolality of the seminal fluid and the capacity of the spermatozoa to fertilize, hatching rate and health of the resulting embryos were examined at different aging times. Whole-genome bisulfite sequencing was used to compare the global and gene-specific DNA methylation in fresh and aged spermatozoa. The results demonstrated that spermatozoa aging in common carp significantly affects their performance and thus the success of artificial fertilization. The methylation level at the cytosine-phosphate-guanine (CpG) sites increased significantly with 24 HPS spermatozoa compared to the fresh group at 1 HPS and then decreased significantly at 96 HPS. A more detailed investigation of gene specific differences in the DNA methylation was hindered by incomplete annotation of the <i>C. carpio</i> genome in the public databases. |
| topic |
fish common carp sperm aging epigenetics DNA methylation sperm quality |
| url |
https://www.mdpi.com/1422-0067/22/11/5925 |
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doaj-70ae96d74e634f7e95007e53aeb1cf8d2021-06-01T01:48:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-01225925592510.3390/ijms22115925Changes in Phenotypes and DNA Methylation of In Vitro Aging Sperm in Common Carp <i>Cyprinus carpio</i>Yu Cheng0Pavlina Vechtova1Zoltan Fussy2Jan Sterba3Zuzana Linhartová4Marek Rodina5Vladimíra Tučková6David Gela7Azin Mohagheghi Samarin8Ievgen Lebeda9Miaomiao Xin10Songpei Zhang11Deepali Rahi12Otomar Linhart13South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicFaculty of Science, Institute of Chemistry and Biochemistry, University of South Bohemia in Ceske Budejovice, Branisovska 1760, 37005 Ceske Budejovice, Czech RepublicFaculty of Science, Institute of Chemistry and Biochemistry, University of South Bohemia in Ceske Budejovice, Branisovska 1760, 37005 Ceske Budejovice, Czech RepublicFaculty of Science, Institute of Chemistry and Biochemistry, University of South Bohemia in Ceske Budejovice, Branisovska 1760, 37005 Ceske Budejovice, Czech RepublicSouth Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicSouth Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicSouth Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicSouth Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicSouth Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicSouth Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicSouth Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicSouth Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicSouth Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicSouth Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in České Budějovice, Zátiší 728/II, 389 25 Vodňany, Czech RepublicThe purpose of the current study was to analyze phenotypic and functional characteristics of common carp (<i>Cyprinus carpio</i>) spermatozoa during in vitro aging and to investigate whether global DNA methylation is affected by sperm aging. Milt was collected from five individual males, stored in vitro on ice in a refrigerator for up to 96 h post stripping (HPS) and used to fertilize eggs with intervals of 1, 24 and 96 h. Computer-assisted sperm analysis and a S3e Cell Sorter was employed to determine the spermatozoa phenotypic characteristics (motility, velocity, concentration and viability). In addition, pH and osmolality of the seminal fluid and the capacity of the spermatozoa to fertilize, hatching rate and health of the resulting embryos were examined at different aging times. Whole-genome bisulfite sequencing was used to compare the global and gene-specific DNA methylation in fresh and aged spermatozoa. The results demonstrated that spermatozoa aging in common carp significantly affects their performance and thus the success of artificial fertilization. The methylation level at the cytosine-phosphate-guanine (CpG) sites increased significantly with 24 HPS spermatozoa compared to the fresh group at 1 HPS and then decreased significantly at 96 HPS. A more detailed investigation of gene specific differences in the DNA methylation was hindered by incomplete annotation of the <i>C. carpio</i> genome in the public databases.https://www.mdpi.com/1422-0067/22/11/5925fishcommon carpsperm agingepigeneticsDNA methylationsperm quality |