DNA methylation of the <i>PLIN1</i> promoter downregulates expression in chicken lines

DNA methylation of the <i>PLIN1</i> promoter downregulates expression in chicken lines

<p>Evidence suggests that Perilipin-1 (<i>PLIN1</i>) is subject to functional regulation by epigenetic modifications in women with obesity. However, whether chicken <i>PLIN1</i> expression is regulated by DNA methylation is unknown. Here, Sequenom MassARRAY and real-tim...

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Main Authors: Y. Sun, R. Li, G. Zhai, X. Zhang, Y. Wang
Format: Article
Language:English
Published: Copernicus Publications 2019-07-01
Series:Archives Animal Breeding
Online Access:https://www.arch-anim-breed.net/62/375/2019/aab-62-375-2019.pdf
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spelling doaj-70ad665f8a8d4e24b74aa13d927572a32020-11-25T02:27:40ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222019-07-016237538210.5194/aab-62-375-2019DNA methylation of the <i>PLIN1</i> promoter downregulates expression in chicken linesY. Sun0Y. Sun1Y. Sun2R. Li3R. Li4R. Li5G. Zhai6G. Zhai7G. Zhai8X. Zhang9X. Zhang10X. Zhang11Y. Wang12Y. Wang13Y. Wang14Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Heilongjiang 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, ChinaKey Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Heilongjiang 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, ChinaKey Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Heilongjiang 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, ChinaKey Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Heilongjiang 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, ChinaKey Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction, Education Department of Heilongjiang Province, Heilongjiang 150030, ChinaCollege of Animal Science and Technology, Northeast Agricultural University, Harbin, Heilongjiang 150030, China<p>Evidence suggests that Perilipin-1 (<i>PLIN1</i>) is subject to functional regulation by epigenetic modifications in women with obesity. However, whether chicken <i>PLIN1</i> expression is regulated by DNA methylation is unknown. Here, Sequenom MassARRAY and real-time polymerase chain reaction (PCR) were conducted to analyze the promoter methylation status and expression of the <i>PLIN1</i> gene in Northeast Agricultural University broiler lines divergently selected for abdominal fat content. We found that chicken <i>PLIN1</i> expression was significantly higher in adipose tissue of fat-line broilers than in lean lines at 1–7 weeks of age, and was significantly positively correlated with abdominal fat percentage (AFP) in chicken adipose development (Pearson's <span class="inline-formula"><i>r</i>=0.627</span>, <span class="inline-formula"><i>P</i>&lt;0.001</span>). The region analyzed for DNA methylation was from <span class="inline-formula">−12</span> to <span class="inline-formula">−520</span>&thinsp;bp upstream of the translation start codon ATG, and had five CpG sites, where only the DNA methylation levels of CpG5 located at position <span class="inline-formula">−490</span>&thinsp;bp were significantly higher in lean compared to fat chickens at 5 and 6 weeks (<span class="inline-formula"><i>P</i>&lt;0.05</span>) and were significantly negatively correlated with <i>PLIN1</i> mRNA levels and AFP (<span class="inline-formula"><i>P</i>&lt;0.05</span>). These results shed new light on the regulation of hypertrophic growth in chicken adipose development.</p>https://www.arch-anim-breed.net/62/375/2019/aab-62-375-2019.pdf
collection DOAJ
language English
format Article
sources DOAJ
author Y. Sun
Y. Sun
Y. Sun
R. Li
R. Li
R. Li
G. Zhai
G. Zhai
G. Zhai
X. Zhang
X. Zhang
X. Zhang
Y. Wang
Y. Wang
Y. Wang
spellingShingle Y. Sun
Y. Sun
Y. Sun
R. Li
R. Li
R. Li
G. Zhai
G. Zhai
G. Zhai
X. Zhang
X. Zhang
X. Zhang
Y. Wang
Y. Wang
Y. Wang
DNA methylation of the <i>PLIN1</i> promoter downregulates expression in chicken lines
Archives Animal Breeding
author_facet Y. Sun
Y. Sun
Y. Sun
R. Li
R. Li
R. Li
G. Zhai
G. Zhai
G. Zhai
X. Zhang
X. Zhang
X. Zhang
Y. Wang
Y. Wang
Y. Wang
author_sort Y. Sun
title DNA methylation of the <i>PLIN1</i> promoter downregulates expression in chicken lines
title_short DNA methylation of the <i>PLIN1</i> promoter downregulates expression in chicken lines
title_full DNA methylation of the <i>PLIN1</i> promoter downregulates expression in chicken lines
title_fullStr DNA methylation of the <i>PLIN1</i> promoter downregulates expression in chicken lines
title_full_unstemmed DNA methylation of the <i>PLIN1</i> promoter downregulates expression in chicken lines
title_sort dna methylation of the <i>plin1</i> promoter downregulates expression in chicken lines
publisher Copernicus Publications
series Archives Animal Breeding
issn 0003-9438
2363-9822
publishDate 2019-07-01
description <p>Evidence suggests that Perilipin-1 (<i>PLIN1</i>) is subject to functional regulation by epigenetic modifications in women with obesity. However, whether chicken <i>PLIN1</i> expression is regulated by DNA methylation is unknown. Here, Sequenom MassARRAY and real-time polymerase chain reaction (PCR) were conducted to analyze the promoter methylation status and expression of the <i>PLIN1</i> gene in Northeast Agricultural University broiler lines divergently selected for abdominal fat content. We found that chicken <i>PLIN1</i> expression was significantly higher in adipose tissue of fat-line broilers than in lean lines at 1–7 weeks of age, and was significantly positively correlated with abdominal fat percentage (AFP) in chicken adipose development (Pearson's <span class="inline-formula"><i>r</i>=0.627</span>, <span class="inline-formula"><i>P</i>&lt;0.001</span>). The region analyzed for DNA methylation was from <span class="inline-formula">−12</span> to <span class="inline-formula">−520</span>&thinsp;bp upstream of the translation start codon ATG, and had five CpG sites, where only the DNA methylation levels of CpG5 located at position <span class="inline-formula">−490</span>&thinsp;bp were significantly higher in lean compared to fat chickens at 5 and 6 weeks (<span class="inline-formula"><i>P</i>&lt;0.05</span>) and were significantly negatively correlated with <i>PLIN1</i> mRNA levels and AFP (<span class="inline-formula"><i>P</i>&lt;0.05</span>). These results shed new light on the regulation of hypertrophic growth in chicken adipose development.</p>
url https://www.arch-anim-breed.net/62/375/2019/aab-62-375-2019.pdf
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