Evaluation of Iraqi Rice Cultivars for Their Tolerance to Drought Stress

• Main Authors: Tiba Nazar Ibrahim Al Azzawi, Murtaza Khan, Adil Hussain, Muhammad Shahid, Qari Muhammad Imran, Bong-Gyu Mun, Sang-Uk Lee, Byung-Wook Yun
• Format: Article
• Language: English
• Published: MDPI AG 2020-11-01
• Series: Agronomy
• Subjects: rice cultivars; drought stress; transcription factors; chlorophyll content; shoot and root length; qRT PCR
• Online Access: https://www.mdpi.com/2073-4395/10/11/1782

Drought stress is a serious problem around the globe and particularly in the Republic of Iraq. Rice is the third most consumed crop for the Iraqi people; however, its cultivation and production is very low due to several challenges including drought. The current study was performed to evaluate five Iraqi rice cultivars along with relevant (drought-tolerant and drought-susceptible) controls under drought stress, either by treatment with 10% PEG (polyethylene glycol) or through water withholding to induce natural drought stress. The phenotypes of all the cultivars were evaluated and the transcriptional responses of key drought-responsive candidate genes, identified through the EST-SSR marker-based approach, were studied. We also studied transcript accumulation of drought-related transcriptional factors, such as <i>OsGRASS23</i>, <i>OsbZIP12,</i> and <i>OsDREB2A.</i> Moreover, the reference cultivars also included a drought-tolerant inter-specific cultivar Nerica 7 (a cross between <i>Oryza sativa</i> ssp. <i>indica</i> X <i>O.</i><i>glaberrima</i>). Among the cultivars, the more drought-tolerant phenotypic characteristics and higher transcript accumulation of drought-related marker genes <i>OsE647</i> and <i>OsE1899</i> and transcriptional factors <i>OsGRASS23</i>, <i>OsbZIP12</i>, and <i>OsDREB2A</i> were observed in four (out of five) significantly drought-tolerant Iraqi cultivars; Mashkab, followed by Furat, Yasmen, and Amber 33. On another note, Amber Barka was found to be significantly drought susceptible. Mashkab and Amber Barka were found to be the most drought-tolerant and-susceptible cultivars, respectively. The identified tolerant cultivars may potentially serve as a genetic source for the incorporation of drought-tolerant phenotypes in rice.