Comparative analysis of five DNA isolation protocols and three drying methods for leaves samples of Nectandra megapotamica (Spreng.) Mez

Comparative analysis of five DNA isolation protocols and three drying methods for leaves samples of Nectandra megapotamica (Spreng.) Mez

The aim of the study was to establish a DNA isolation protocol Nectandra megapotamica (Spreng.) Mez., able to obtain samples of high yield and quality for use in genomic analysis. A commercial kit and four classical methods of DNA extraction were tested, including three cetyltrimethylammonium bromid...

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Bibliographic Details
Main Authors: Leonardo Severo da Costa, Lia Rejane Silveira Reiniger, Valdir Marcos Stefenon, Berta Maria Heinzmann, Adriel dos Santos Oliveira
Format: Article
Language:English
Published: Universidade Estadual de Londrina 2016-06-01
Series:Semina: Ciências Agrárias
Subjects:
CTAB
SDS
Cost
Quality
Yield.
Online Access:http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/21305

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