Control of Ralstonia solanacearum Infection in Tomato, Brinjal and Capsicum by antibiotic sensitivity test

Ralstonia solanacearum is one of the important dreaded soil’s borne bacterial phytopathogen which causes enormous losses in the crop plants in tropical, subtropical and temperate region of the world. In India, the disease is highly prevalent and active throughout the year where the soil is acidic i...

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Main Authors: Rupa Verma, Abhijit Dutta, Ashok Kumar Choudhary, Sudarshan Maurya
Format: Article
Language:English
Published: Society of Open Science 2014-07-01
Series:Journal of Advanced Laboratory Research in Biology
Subjects:
Online Access:https://e-journal.sospublication.co.in/index.php/jalrb/article/view/184
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spelling doaj-706551b6f3bd41a7b0f09708cfd80fa72020-11-25T00:48:55ZengSociety of Open ScienceJournal of Advanced Laboratory Research in Biology0976-76142014-07-0153Control of Ralstonia solanacearum Infection in Tomato, Brinjal and Capsicum by antibiotic sensitivity testRupa Verma0Abhijit Dutta1Ashok Kumar Choudhary2Sudarshan Maurya3Department of Botany, Ranchi University, Ranchi - 834008, Jharkhand, India.Department of Zoology, Ranchi University, Ranchi - 834008, Jharkhand, India.Department of Botany, Ranchi University, Ranchi - 834008, Jharkhand, India.ICAR-Research Complex for Eastern Region, Research Center, Ranchi - 834001, India. Ralstonia solanacearum is one of the important dreaded soil’s borne bacterial phytopathogen which causes enormous losses in the crop plants in tropical, subtropical and temperate region of the world. In India, the disease is highly prevalent and active throughout the year where the soil is acidic in the Eastern Plateau and Hill Region. Once the disease is established in the field, it cannot be controlled by chemical means. Keeping these in view, screening of potential antibiotics for the management of R. solanacearum was done. In the present study, three strains of R. solanacearum were isolated from Brinjal, Tomato and Capsicum. Against these three strains of R. solanacearum, four antibiotics were screened through food poison techniques viz. Kasugamycin, Streptomycin, Ceftriaxone and Gentamicin. The different strains of R. solanacearum and antibiotic sensitivity showed varied response. Among the screened antibiotics, Gentamicin showed strong antibacterial efficacy which inhibited 100% Colony-forming Unit (cfu) at very low concentration (1 ppm) followed by Ceftriaxone which inhibited >50% cfu at 1 ppm against all three strains. However, Streptomycin also showed antibacterial efficacy and inhibited >50% cfu at 3 ppm, but Kasugamycin was found less antibacterial as compared to other tested antibiotics and inhibited >50% cfu at 4 ppm. https://e-journal.sospublication.co.in/index.php/jalrb/article/view/184Bacterial wiltAntibioticsRalstonia solanacearumantibacterial
collection DOAJ
language English
format Article
sources DOAJ
author Rupa Verma
Abhijit Dutta
Ashok Kumar Choudhary
Sudarshan Maurya
spellingShingle Rupa Verma
Abhijit Dutta
Ashok Kumar Choudhary
Sudarshan Maurya
Control of Ralstonia solanacearum Infection in Tomato, Brinjal and Capsicum by antibiotic sensitivity test
Journal of Advanced Laboratory Research in Biology
Bacterial wilt
Antibiotics
Ralstonia solanacearum
antibacterial
author_facet Rupa Verma
Abhijit Dutta
Ashok Kumar Choudhary
Sudarshan Maurya
author_sort Rupa Verma
title Control of Ralstonia solanacearum Infection in Tomato, Brinjal and Capsicum by antibiotic sensitivity test
title_short Control of Ralstonia solanacearum Infection in Tomato, Brinjal and Capsicum by antibiotic sensitivity test
title_full Control of Ralstonia solanacearum Infection in Tomato, Brinjal and Capsicum by antibiotic sensitivity test
title_fullStr Control of Ralstonia solanacearum Infection in Tomato, Brinjal and Capsicum by antibiotic sensitivity test
title_full_unstemmed Control of Ralstonia solanacearum Infection in Tomato, Brinjal and Capsicum by antibiotic sensitivity test
title_sort control of ralstonia solanacearum infection in tomato, brinjal and capsicum by antibiotic sensitivity test
publisher Society of Open Science
series Journal of Advanced Laboratory Research in Biology
issn 0976-7614
publishDate 2014-07-01
description Ralstonia solanacearum is one of the important dreaded soil’s borne bacterial phytopathogen which causes enormous losses in the crop plants in tropical, subtropical and temperate region of the world. In India, the disease is highly prevalent and active throughout the year where the soil is acidic in the Eastern Plateau and Hill Region. Once the disease is established in the field, it cannot be controlled by chemical means. Keeping these in view, screening of potential antibiotics for the management of R. solanacearum was done. In the present study, three strains of R. solanacearum were isolated from Brinjal, Tomato and Capsicum. Against these three strains of R. solanacearum, four antibiotics were screened through food poison techniques viz. Kasugamycin, Streptomycin, Ceftriaxone and Gentamicin. The different strains of R. solanacearum and antibiotic sensitivity showed varied response. Among the screened antibiotics, Gentamicin showed strong antibacterial efficacy which inhibited 100% Colony-forming Unit (cfu) at very low concentration (1 ppm) followed by Ceftriaxone which inhibited >50% cfu at 1 ppm against all three strains. However, Streptomycin also showed antibacterial efficacy and inhibited >50% cfu at 3 ppm, but Kasugamycin was found less antibacterial as compared to other tested antibiotics and inhibited >50% cfu at 4 ppm.
topic Bacterial wilt
Antibiotics
Ralstonia solanacearum
antibacterial
url https://e-journal.sospublication.co.in/index.php/jalrb/article/view/184
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