Disparate Phenotypes Resulting from Mutations of a Single Histidine in Switch II of <i>Geobacillus stearothermophilus</i> Translation Initiation Factor IF2

Disparate Phenotypes Resulting from Mutations of a Single Histidine in Switch II of <i>Geobacillus stearothermophilus</i> Translation Initiation Factor IF2

The conserved Histidine 301 in switch II of <i>Geobacillus stearothermophilus</i> IF2 G2 domain was substituted with Ser, Gln, Arg, Leu and Tyr to generate mutants displaying different phenotypes. Overexpression of IF2H301S, IF2H301L and IF2H301Y in cells expressing wtIF2, unlike IF2H301...

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Bibliographic Details
Main Authors: Jerneja Tomsic, Arianna Smorlesi, Enrico Caserta, Anna Maria Giuliodori, Cynthia L. Pon, Claudio O. Gualerzi
Format: Article
Language:English
Published: MDPI AG 2020-01-01
Series:International Journal of Molecular Sciences
Subjects:
protein synthesis
translation initiation
if2 structure
switch ii mutations
if2 recycling
gtp hydrolysis
Online Access:https://www.mdpi.com/1422-0067/21/3/735

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