The application of next-generation sequence-based DNA barcoding for bloodmeal detection in host-seeking wild-caught Ixodes scapularis nymphs

The application of next-generation sequence-based DNA barcoding for bloodmeal detection in host-seeking wild-caught Ixodes scapularis nymphs

Abstract Objective Our objective was to apply next-generation sequence-based DNA barcoding to identify the remnant larval bloodmeals in wild-caught host-seeking (unengorged) Ixodes scapularis nymphs (n = 216). To infer host species identification, vertebrate DNA was amplified using universal primers...

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Bibliographic Details
Main Authors: G. A. Lumsden, E. V. Zakharov, S. Dolynskyj, J. S. Weese, L. R. Lindsay, C. M. Jardine
Format: Article
Language:English
Published: BMC 2021-02-01
Series:BMC Research Notes
Subjects:
Bloodmeal analysis
DNA barcoding
Blacklegged tick
Host identification
Next-generation sequencing
Online Access:https://doi.org/10.1186/s13104-021-05481-3

Internet

https://doi.org/10.1186/s13104-021-05481-3

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