Biochemical issues in estimation of cytosolic free NAD/NADH ratio.

Biochemical issues in estimation of cytosolic free NAD/NADH ratio.

Cytosolic free NAD/NADH ratio is fundamentally important in maintaining cellular redox homeostasis but current techniques cannot distinguish between protein-bound and free NAD/NADH. Williamson et al reported a method to estimate this ratio by cytosolic lactate/pyruvate (L/P) based on the principle o...

Full description

Bibliographic Details
Main Authors:	Feifei Sun, Chunyan Dai, Jiansheng Xie, Xun Hu
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2012-01-01
Series:	PLoS ONE
Online Access:	http://europepmc.org/articles/PMC3343042?pdf=render

Similar Items

Modelling the impact of changes in the extracellular environment on the cytosolic free NAD+/NADH ratio during cell culture.
by: Ross A Kelly, et al.
Published: (2018-01-01)

Ca2+ dependence of gluconeogenesis stimulation by glucagon at different cytosolic NAD+-NADH redox potentials
by: A.C. Marques-da-Silva, et al.
Published: (1997-07-01)

Cofactor engineering to regulate NAD+/NADH ratio with its application to phytosterols biotransformation
by: Liqiu Su, et al.
Published: (2017-10-01)

Methotrexate use and NAD+/NADH metabolism in psoriatic keratinocytes
by: Agata Liszewska, et al.
Published: (2020-03-01)

A novel cytosolic NADH:quinone oxidoreductase from Methanothermobacter marburgensis
by: Eva Ullmann, et al.
Published: (2014-12-01)

Correlations of mitochondrial morphology and [NADH]/[NAD+] levels in Saccharomyces cerevisiae
by: CHUNG-TING LU, et al.
Published: (2019)

Utilizing NAD+/NADH Analogs for the Solar Fuel Forming Reductions
by: Ilic, Stefan
Published: (2017)

Nutritional Ketosis Increases NAD+/NADH Ratio in Healthy Human Brain: An in Vivo Study by 31P-MRS
by: Lijing Xin, et al.
Published: (2018-07-01)

Tolerance to NADH/NAD+ imbalance anticipates aging and anti-aging interventions
by: Alvar J. Alonso-Lavin, et al.
Published: (2021-07-01)

Sources and implications of NADH/NAD+ redox imbalance in diabetes and its complications
by: Wu J, et al.
Published: (2016-05-01)

NADH/NAD<sup>+</sup> Redox Imbalance and Diabetic Kidney Disease
by: Liang-Jun Yan
Published: (2021-05-01)

UVB-311 nm phototherapy and NAD(+)/NADH metabolism in keratinocytes in patients with psoriasis
by: Małgorzata Bernacka, et al.
Published: (2019-01-01)

Electrochemical investigations of the interactive behavior of Nicotinamide Adenine Dinucleotide (NAD+/NADH) with electrode surfaces: towards direct electrochemical regeneration of enzymatically-active NADH
by: Ali, Irshad
Published: (2013)

Toxicity of Melaleuca alternifolia essential oil to the mitochondrion and NAD+/NADH dehydrogenase in Tribolium confusum
by: Min Liao, et al.
Published: (2018-11-01)

Imaging the NADH:NAD+ homeostasis for understanding the metabolic response of Mycobacterium to physiologically relevant stresses
by: Shabir Ahmad Bhat, et al.
Published: (2016-11-01)

In planta study of photosynthesis and photorespiration using NADPH and NADH/NAD+ fluorescent protein sensors
by: Shey-Li Lim, et al.
Published: (2020-06-01)

Phenothiazine normalizes the NADH/NAD+ ratio, maintains mitochondrial integrity and protects the nigrostriatal dopamine system in a chronic rotenone model of Parkinson's disease
by: Victor Tapias, et al.
Published: (2019-06-01)

Cloning and expression of mitochondrial-encoded subunits Nad1, Nad4 and Nad5 of complex I (NADH:ubiquinone oxidoreductase) from Bambusa oldhamii and Phyllostachys edulis
by: Hsieh-Chin Tsai, et al.
Published: (2009)

Using Fractional Intensities of Time-resolved Fluorescence to Sensitively Quantify NADH/NAD+ with Genetically Encoded Fluorescent Biosensors
by: Mengfang Chang, et al.
Published: (2017-06-01)

Bioinspired Design of Alcohol Dehydrogenase@nano TiO2 Microreactors for Sustainable Cycling of NAD+/NADH Coenzyme
by: Sen Lin, et al.
Published: (2018-02-01)

The activity of NADH-, NADPH- and Fd-dependent glutamate synthase in the plastids and cytosol of Pisum arvense L. root cells
by: Genowefa Kubik-Dobosz
Published: (2014-01-01)

Highly efficient 5' capping of mitochondrial RNA with NAD+ and NADH by yeast and human mitochondrial RNA polymerase
by: Jeremy G Bird, et al.
Published: (2018-12-01)

NAD+/NADH redox alterations reconfigure metabolism and rejuvenate senescent human mesenchymal stem cells in vitro
by: Xuegang Yuan, et al.
Published: (2020-12-01)

COFACTOR SPECIFICITY ENGINEERING OF STREPTOCOCCUS MUTANS NADH OXIDASE 2 FOR NAD(P)+ REGENERATION IN BIOCATALYTIC OXIDATIONS
by: Barbara Petschacher, et al.
Published: (2014-02-01)

Catalysis of Mitochondrial NADH→NAD<sup>+</sup> Transhydrogenation in Adult <i>Ascaris suum</i> (Nematoda)
by: Holowiecki, Andrew
Published: (2009)

Neuroprotection in Glaucoma: NAD<sup>+</sup>/NADH Redox State as a Potential Biomarker and Therapeutic Target
by: Bledi Petriti, et al.
Published: (2021-06-01)

Oxygen reduction activity of carbon fibers cathode, and application for current generation from the NAD+ and NADH recycling reaction
by: H. Maeda, et al.
Published: (2012-03-01)

Ectopic Expression of Rv0023 Mediates Isoniazid/Ethionamide Tolerance via Altering NADH/NAD+ Levels in Mycobacterium smegmatis
by: Shailesh Kumar Gupta, et al.
Published: (2020-02-01)

Creation of an allosteric site and a NAD+ binding site in cytosolic NADP+-dependent malic enzyme.
by: Meng-Chun Chen, et al.
Published: (2008)

Régulation de la fonction mitochondriale par le rapport NADH/NAD+ : le rôle clef du complexe I
by: Leman, Géraldine
Published: (2014)

Regulation of bile acid 7-dehydroxylase activity by NAD+ and NADH in cell extracts of Eubacterium species V.P.I. 12708
by: B A White, et al.
Published: (1983-01-01)

Altered Expression of Mitochondrial NAD+ Carriers Influences Yeast Chronological Lifespan by Modulating Cytosolic and Mitochondrial Metabolism
by: Ivan Orlandi, et al.
Published: (2018-12-01)

New biotechnological perspectives of a NADH oxidase variant from <it>Thermus thermophilus </it>HB27 as NAD<sup>+</sup>-recycling enzyme
by: Rocha-Martín Javier, et al.
Published: (2011-11-01)

Redox specificity of 2-hydroxyacid-coupled NAD(+)/NADH dehydrogenases: a study exploiting "reactive" arginine as a reporter of protein electrostatics.
by: Pooja Gupta, et al.
Published: (2013-01-01)

Impairment of NADH dehydrogenase and regulation of anaerobic metabolism by the small RNA RyhB and NadE for improved biohydrogen production in Enterobacter aerogenes
by: Yan Wu, et al.
Published: (2017-10-01)

Cytosolic signalling and behaviour of oral neutrophils “Search for biochemical memory”
by: Elumalai, Geetha Lakshmi
Published: (2012)

Hepatic steatosis induced in C57BL/6 mice by a non-ß oxidizable fatty acid analogue is associated with reduced plasma kynurenine metabolites and a modified hepatic NAD+/NADH ratio
by: Rolf K. Berge, et al.
Published: (2020-05-01)

Correction: Redox Specificity of 2-Hydroxyacid-Coupled NAD+/NADH Dehydrogenases: A Study Exploiting "Reactive" Arginine as a Reporter of Protein Electrostatics.
by: Pooja Gupta, et al.
Published: (2016-01-01)

Studies of NADH oxidation by NADH : ubiquinone oxidoreductase (complex I) from bovine mitochondria
by: Barker, C. D.
Published: (2007)

Developments in flourescence measurements of NADH
by: Haramis, Helena
Published: (2003)