Exploration of microorganisms in hospital operating room, university classroom and meeting room by modified sampling collector compared to open dish method

• Main Authors: Pallop Tankaew, Phanita Komolmal, Jongkon Jainoonwong, Proawpilart Intayot, Bongkotwan Sutabhaha
• Format: Article
• Language: English
• Published: Chaing Mai University 2009-01-01
• Series: Journal of Associated Medical Sciences
• Subjects: air borne microorganism collector; air borne microorganisms in special control room of the hospital and faculty building
• Online Access: https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/60093

Six locations aroud the room and four corners of operating bed in hospital operating room were investigated for microorganisms using modified sample collector. The result showed that at least 8 genus of fungi (Aspergillus sp., Cladosporium sp., Mucor sp., Rhizopus sp., Penicillium sp., Scopulariopsis sp., non sporulating fungi, Yeasts) and 6 genus of bacteria (Staph. saprophyticus, Staph. epidermidis, Micrococcus sp., Bacillus sp., Flavobacterium sp. and Diphtheroid sp.) were found .The floor cleaning after operation with anti-infectious agent mopping could not decrease the total number of fungi (333/301, 139/172) or bacteria (372/471, 196/254) both in locations around the room and the corners of operation bed. The same results were found in university classroom with at least 7 genus of fungi (Aspergillus niger., Aspergillus sp., Cladosporium sp., Rhizopus sp., Penicillium sp., Fusarium sp., non sporulating fungi) and 3 genus of bacteria (Staph.coagulase neg., Bacillus sp., Acinetobacter lwoffii) were found and after mopping, the total number of fungi (333/301, 139/172) and bacteria (372/471, 196/254) did not decrease. After changing the cleaning method by using anti-dust agent mopping floor of classroom, the result showed 5 times decreasing in the number of fungi (8/43). In addition, comparing to open disk method, the modified sample collector could collect more genus and more number in every exporation. Bull Chiang Mai Assoc Sci 2009; 42: 25-36.