In Vitro Sonodynamic Therapeutic Effect of Polyion Complex Micelles Incorporating Titanium Dioxide Nanoparticles

In Vitro Sonodynamic Therapeutic Effect of Polyion Complex Micelles Incorporating Titanium Dioxide Nanoparticles

Titanium dioxide nanoparticles (TiO2 NPs) can act as sonosensitizers, generating reactive oxygen species under ultrasound irradiation, for use in sonodynamic therapy. For TiO2 NPs delivery, we prepared polyion complex micelles incorporating TiO2 NPs (TiO2 NPs-PIC micelles) by mixing TiO2 NPs with po...

Full description

Bibliographic Details
Main Authors: Satoshi Yamamoto, Masafumi Ono, Eiji Yuba, Atsushi Harada
Format: Article
Language:English
Published: MDPI AG 2017-09-01
Series:Nanomaterials
Subjects:
Titanium dioxide nanoparticles
sonodynamic therapy
polyion complex micelles
Online Access:https://www.mdpi.com/2079-4991/7/9/268
id doaj-6f92e891e37f4474a9026371f498c64f
record_format Article
spelling doaj-6f92e891e37f4474a9026371f498c64f2020-11-25T00:47:13ZengMDPI AGNanomaterials2079-49912017-09-017926810.3390/nano7090268nano7090268In Vitro Sonodynamic Therapeutic Effect of Polyion Complex Micelles Incorporating Titanium Dioxide NanoparticlesSatoshi Yamamoto0Masafumi Ono1Eiji Yuba2Atsushi Harada3Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8531, JapanTitanium dioxide nanoparticles (TiO2 NPs) can act as sonosensitizers, generating reactive oxygen species under ultrasound irradiation, for use in sonodynamic therapy. For TiO2 NPs delivery, we prepared polyion complex micelles incorporating TiO2 NPs (TiO2 NPs-PIC micelles) by mixing TiO2 NPs with polyallylamine bearing poly(ethylene glycol) grafts. In this study, the effects of polymer composition and ultrasound irradiation conditions on the sonodynamic therapeutic effect toward HeLa cells were evaluated experimentally using cell viability evaluation, intracellular distribution observation, and a cell staining assay. TiO2 NPs-PIC micelles with widely distributed features induced a significant decrease in cell viability under ultrasound irradiation. Furthermore, prolonging the irradiation time killed cells more effectively than did increasing the ultrasound power. The combination of TiO2 NP-PIC micelles and ultrasound irradiation was confirmed to induce apoptotic cell death.https://www.mdpi.com/2079-4991/7/9/268Titanium dioxide nanoparticlessonodynamic therapypolyion complex micelles
collection DOAJ
language English
format Article
sources DOAJ
author Satoshi Yamamoto
Masafumi Ono
Eiji Yuba
Atsushi Harada
spellingShingle Satoshi Yamamoto
Masafumi Ono
Eiji Yuba
Atsushi Harada
In Vitro Sonodynamic Therapeutic Effect of Polyion Complex Micelles Incorporating Titanium Dioxide Nanoparticles
Nanomaterials
Titanium dioxide nanoparticles
sonodynamic therapy
polyion complex micelles
author_facet Satoshi Yamamoto
Masafumi Ono
Eiji Yuba
Atsushi Harada
author_sort Satoshi Yamamoto
title In Vitro Sonodynamic Therapeutic Effect of Polyion Complex Micelles Incorporating Titanium Dioxide Nanoparticles
title_short In Vitro Sonodynamic Therapeutic Effect of Polyion Complex Micelles Incorporating Titanium Dioxide Nanoparticles
title_full In Vitro Sonodynamic Therapeutic Effect of Polyion Complex Micelles Incorporating Titanium Dioxide Nanoparticles
title_fullStr In Vitro Sonodynamic Therapeutic Effect of Polyion Complex Micelles Incorporating Titanium Dioxide Nanoparticles
title_full_unstemmed In Vitro Sonodynamic Therapeutic Effect of Polyion Complex Micelles Incorporating Titanium Dioxide Nanoparticles
title_sort in vitro sonodynamic therapeutic effect of polyion complex micelles incorporating titanium dioxide nanoparticles
publisher MDPI AG
series Nanomaterials
issn 2079-4991
publishDate 2017-09-01
description Titanium dioxide nanoparticles (TiO2 NPs) can act as sonosensitizers, generating reactive oxygen species under ultrasound irradiation, for use in sonodynamic therapy. For TiO2 NPs delivery, we prepared polyion complex micelles incorporating TiO2 NPs (TiO2 NPs-PIC micelles) by mixing TiO2 NPs with polyallylamine bearing poly(ethylene glycol) grafts. In this study, the effects of polymer composition and ultrasound irradiation conditions on the sonodynamic therapeutic effect toward HeLa cells were evaluated experimentally using cell viability evaluation, intracellular distribution observation, and a cell staining assay. TiO2 NPs-PIC micelles with widely distributed features induced a significant decrease in cell viability under ultrasound irradiation. Furthermore, prolonging the irradiation time killed cells more effectively than did increasing the ultrasound power. The combination of TiO2 NP-PIC micelles and ultrasound irradiation was confirmed to induce apoptotic cell death.
topic Titanium dioxide nanoparticles
sonodynamic therapy
polyion complex micelles
url https://www.mdpi.com/2079-4991/7/9/268
work_keys_str_mv AT satoshiyamamoto invitrosonodynamictherapeuticeffectofpolyioncomplexmicellesincorporatingtitaniumdioxidenanoparticles
AT masafumiono invitrosonodynamictherapeuticeffectofpolyioncomplexmicellesincorporatingtitaniumdioxidenanoparticles
AT eijiyuba invitrosonodynamictherapeuticeffectofpolyioncomplexmicellesincorporatingtitaniumdioxidenanoparticles
AT atsushiharada invitrosonodynamictherapeuticeffectofpolyioncomplexmicellesincorporatingtitaniumdioxidenanoparticles
_version_ 1725261228235816960

Similar Items