Monitoring <i>E. coli</i> Cell Integrity by ATR-FTIR Spectroscopy and Chemometrics: Opportunities and Caveats
During recombinant protein production with <i>E. coli</i>, the integrity of the inner and outer membrane changes, which leads to product leakage (loss of outer membrane integrity) or lysis (loss of inner membrane integrity). Motivated by current Quality by Design guidelines, there is a n...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
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| Series: | Processes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9717/9/3/422 |