Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples

Synthesis and Evaluation of a Molecularly Imprinted Polymer for Selective Solid-Phase Extraction of Irinotecan from Human Serum Samples

A molecularly imprinted polymer (MIP) was synthesized by non-covalent imprinting polymerization using irinotecan as template. Methacrylic acid and 4-vinylpyridine were selected as functional monomers. An optimized procedure coupled to LC-PDA analysis was developed for the selective solid-phase extra...

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Bibliographic Details
Main Authors: Isabelle Lefebvre-Tournier, Christian Périgaud, Jérome Guitton, Charles Dumontet, Jean-Yves Puy, Charlotte Martin, Sung Vo Duy, Béatrice Roy
Format: Article
Language:English
Published: MDPI AG 2012-02-01
Series:Journal of Functional Biomaterials
Subjects:
molecularly imprinted polymer
irinotecan
human serum
LC-PDA
Online Access:http://www.mdpi.com/2079-4983/3/1/131/
Description
Summary:A molecularly imprinted polymer (MIP) was synthesized by non-covalent imprinting polymerization using irinotecan as template. Methacrylic acid and 4-vinylpyridine were selected as functional monomers. An optimized procedure coupled to LC-PDA analysis was developed for the selective solid-phase extraction of irinotecan from various organic media. A specific capacity of 0.65 µmol•g−1 for the MIP was determined. The high specificity of this MIP was demonstrated by studying the retention behaviour of two related compounds, camptothecin and SN-38. This support was applied for the extraction of irinotecan from human serum samples.
ISSN:2079-4983

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