An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR

An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR

An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study,...

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Bibliographic Details
Main Authors: Wendell Jacinto Pereira, Priscila Zaczuk Bassinello, Claudio Brondani, Rosana Pereira Vianello
Format: Article
Language:English
Published: Brazilian Society of Plant Breeding 2017-04-01
Series:Crop Breeding and Applied Biotechnology
Subjects:
Molecular Breeding
Gene expression
Gene normalization
Grain RNA extraction
Phaseolus vulgaris
Online Access:http://www.sbmp.org.br/cbab/siscbab/uploads/c8eb9792-a7a4-49db.pdf

Internet

http://www.sbmp.org.br/cbab/siscbab/uploads/c8eb9792-a7a4-49db.pdf

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