Detection of Clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin

Summary: Tetanus resulting from ear injury remains an important health problem, particularly in the developing world. We report the successful detection of Clostridium tetani using tetX specific primers targeting the Cl. tetani neurotoxin. The sample was obtained from an ear discharge of a case of o...

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Main Authors:	Madhu Ganesh, Nasira K. Sheikh, Pooja Shah, Gajanan Mehetre, Mahesh S. Dharne, Basavraj S. Nagoba
Format:	Article
Language:	English
Published:	Elsevier 2016-01-01
Series:	Journal of Infection and Public Health
Online Access:	http://www.sciencedirect.com/science/article/pii/S1876034115001410

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spelling	doaj-6ed125ba9558431b9ed235c6733251e42020-11-24T21:34:00ZengElsevierJournal of Infection and Public Health1876-03412016-01-0191105109Detection of Clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxinMadhu Ganesh0Nasira K. Sheikh1Pooja Shah2Gajanan Mehetre3Mahesh S. Dharne4Basavraj S. Nagoba5Dr. VM Medical College, Solapur 413 001, IndiaDr. VM Medical College, Solapur 413 001, IndiaDr. VM Medical College, Solapur 413 001, IndiaNCIM Resource Centre, CSIR – National Chemical Laboratory (NCL), Pune 411 008, Maharashtra, IndiaNCIM Resource Centre, CSIR – National Chemical Laboratory (NCL), Pune 411 008, Maharashtra, IndiaMaharashtra Institute of Medical Sciences & Research, Latur 413 531, Maharashtra, India; Corresponding author. Tel.: +91 2382227424; fax: +91 2382227246; mobile: +91 9423075786/7588237531.Summary: Tetanus resulting from ear injury remains an important health problem, particularly in the developing world. We report the successful detection of Clostridium tetani using tetX specific primers targeting the Cl. tetani neurotoxin. The sample was obtained from an ear discharge of a case of otogenic tetanus in a 2-year-old male child. Based on the culture results of the ear discharge, Gram staining and virulence testing by genotyping, a diagnosis of tetanus was confirmed. This is the first report from India on the successful detection of Cl. tetani in a human clinical sample using tetX specific primers targeting the Cl. tetani neurotoxin. Keywords: Otogenic tetanus, Cl. tetani, Neurotoxin, Trismushttp://www.sciencedirect.com/science/article/pii/S1876034115001410
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Madhu Ganesh Nasira K. Sheikh Pooja Shah Gajanan Mehetre Mahesh S. Dharne Basavraj S. Nagoba
spellingShingle	Madhu Ganesh Nasira K. Sheikh Pooja Shah Gajanan Mehetre Mahesh S. Dharne Basavraj S. Nagoba Detection of Clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin Journal of Infection and Public Health
author_facet	Madhu Ganesh Nasira K. Sheikh Pooja Shah Gajanan Mehetre Mahesh S. Dharne Basavraj S. Nagoba
author_sort	Madhu Ganesh
title	Detection of Clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin
title_short	Detection of Clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin
title_full	Detection of Clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin
title_fullStr	Detection of Clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin
title_full_unstemmed	Detection of Clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin
title_sort	detection of clostridium tetani in human clinical samples using tetx specific primers targeting the neurotoxin
publisher	Elsevier
series	Journal of Infection and Public Health
issn	1876-0341
publishDate	2016-01-01
description	Summary: Tetanus resulting from ear injury remains an important health problem, particularly in the developing world. We report the successful detection of Clostridium tetani using tetX specific primers targeting the Cl. tetani neurotoxin. The sample was obtained from an ear discharge of a case of otogenic tetanus in a 2-year-old male child. Based on the culture results of the ear discharge, Gram staining and virulence testing by genotyping, a diagnosis of tetanus was confirmed. This is the first report from India on the successful detection of Cl. tetani in a human clinical sample using tetX specific primers targeting the Cl. tetani neurotoxin. Keywords: Otogenic tetanus, Cl. tetani, Neurotoxin, Trismus
url	http://www.sciencedirect.com/science/article/pii/S1876034115001410
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Detection of Clostridium tetani in human clinical samples using tetX specific primers targeting the neurotoxin

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