Characterization of Acid-Aluminium Sensitive Mutants of Soybean Symbiont Bradyrhizobium japonicum Generated by Transposon Mutagenesis

Acid-aluminium sensitive mutants of symbiotic bacterium Bradyrhizobium japonicum BJ11 (designated as AAS11) and KDR15 (designated as AAS15) were constructed by mini-Tn5 transposon mutagenesis to study genes involved in acid-aluminium tolerance (AAT) in B. japonicum. Transposon delivery was carried o...

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Main Authors: ARIS TRI WAHYUDI, ANDINI PURNAWIJAYA, DINI NURDIANI, TEDJA IMAS
Format: Article
Language:English
Published: Indonesian Society for Microbiology 2010-08-01
Series:Microbiology Indonesia
Subjects:
Online Access:https://jurnal.permi.or.id/index.php/mionline/article/view/65
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spelling doaj-6e9312cf08174bf097dec2f06d6be4c72021-08-31T13:00:37ZengIndonesian Society for MicrobiologyMicrobiology Indonesia1978-34772087-85752010-08-011210.5454/mi.1.2.761Characterization of Acid-Aluminium Sensitive Mutants of Soybean Symbiont Bradyrhizobium japonicum Generated by Transposon MutagenesisARIS TRI WAHYUDIANDINI PURNAWIJAYADINI NURDIANITEDJA IMASAcid-aluminium sensitive mutants of symbiotic bacterium Bradyrhizobium japonicum BJ11 (designated as AAS11) and KDR15 (designated as AAS15) were constructed by mini-Tn5 transposon mutagenesis to study genes involved in acid-aluminium tolerance (AAT) in B. japonicum. Transposon delivery was carried out through conjugation between B. japonicum strains as recipients and Escherichia coli S17-1 (ë pir) carrying pUTmini- Tn5Km1 as a donor strain. The result showed that frequency of transconjugation was in the range of 6.7 x 10-7 to 7.1 x 10-6 cell per recipients. AAS11 and AAS15 mutants did not grow on Ayanaba media (pH 4.5) containing 50 μM Aluminium. These mutants remained able to form root nodules of Siratro (Macroptilium arthropurpureum) plants revealing genes interrupted by transposon which were responsible for acid-Al tolerance did not correlate with the nodulation genes. Strains tolerant to acid-aluminium and their mutants with a wild type sensitive to acidaluminium were characterized by accumulating phosphate and aluminium absorption. Compared to the wild type acid-aluminium tolerant B. japonicum, there was approximately a three- to eight-times decrease in phosphate accumulation and a five- to seven-times increase in aluminium absorption by these mutants. These results suggest that aluminium and phosphate contents in the bacterial cells may be involved in mechanisms of acid-Al tolerance of B. japonicum grown in acid-aluminium stress conditions.https://jurnal.permi.or.id/index.php/mionline/article/view/65Bradyrhizobium japonicumacid-aluminium tolerancetransposon mutagenesisaluminium absorptionphosphate intracellular
collection DOAJ
language English
format Article
sources DOAJ
author ARIS TRI WAHYUDI
ANDINI PURNAWIJAYA
DINI NURDIANI
TEDJA IMAS
spellingShingle ARIS TRI WAHYUDI
ANDINI PURNAWIJAYA
DINI NURDIANI
TEDJA IMAS
Characterization of Acid-Aluminium Sensitive Mutants of Soybean Symbiont Bradyrhizobium japonicum Generated by Transposon Mutagenesis
Microbiology Indonesia
Bradyrhizobium japonicum
acid-aluminium tolerance
transposon mutagenesis
aluminium absorption
phosphate intracellular
author_facet ARIS TRI WAHYUDI
ANDINI PURNAWIJAYA
DINI NURDIANI
TEDJA IMAS
author_sort ARIS TRI WAHYUDI
title Characterization of Acid-Aluminium Sensitive Mutants of Soybean Symbiont Bradyrhizobium japonicum Generated by Transposon Mutagenesis
title_short Characterization of Acid-Aluminium Sensitive Mutants of Soybean Symbiont Bradyrhizobium japonicum Generated by Transposon Mutagenesis
title_full Characterization of Acid-Aluminium Sensitive Mutants of Soybean Symbiont Bradyrhizobium japonicum Generated by Transposon Mutagenesis
title_fullStr Characterization of Acid-Aluminium Sensitive Mutants of Soybean Symbiont Bradyrhizobium japonicum Generated by Transposon Mutagenesis
title_full_unstemmed Characterization of Acid-Aluminium Sensitive Mutants of Soybean Symbiont Bradyrhizobium japonicum Generated by Transposon Mutagenesis
title_sort characterization of acid-aluminium sensitive mutants of soybean symbiont bradyrhizobium japonicum generated by transposon mutagenesis
publisher Indonesian Society for Microbiology
series Microbiology Indonesia
issn 1978-3477
2087-8575
publishDate 2010-08-01
description Acid-aluminium sensitive mutants of symbiotic bacterium Bradyrhizobium japonicum BJ11 (designated as AAS11) and KDR15 (designated as AAS15) were constructed by mini-Tn5 transposon mutagenesis to study genes involved in acid-aluminium tolerance (AAT) in B. japonicum. Transposon delivery was carried out through conjugation between B. japonicum strains as recipients and Escherichia coli S17-1 (ë pir) carrying pUTmini- Tn5Km1 as a donor strain. The result showed that frequency of transconjugation was in the range of 6.7 x 10-7 to 7.1 x 10-6 cell per recipients. AAS11 and AAS15 mutants did not grow on Ayanaba media (pH 4.5) containing 50 μM Aluminium. These mutants remained able to form root nodules of Siratro (Macroptilium arthropurpureum) plants revealing genes interrupted by transposon which were responsible for acid-Al tolerance did not correlate with the nodulation genes. Strains tolerant to acid-aluminium and their mutants with a wild type sensitive to acidaluminium were characterized by accumulating phosphate and aluminium absorption. Compared to the wild type acid-aluminium tolerant B. japonicum, there was approximately a three- to eight-times decrease in phosphate accumulation and a five- to seven-times increase in aluminium absorption by these mutants. These results suggest that aluminium and phosphate contents in the bacterial cells may be involved in mechanisms of acid-Al tolerance of B. japonicum grown in acid-aluminium stress conditions.
topic Bradyrhizobium japonicum
acid-aluminium tolerance
transposon mutagenesis
aluminium absorption
phosphate intracellular
url https://jurnal.permi.or.id/index.php/mionline/article/view/65
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