Identification of novel antibody-reactive detection sites for comprehensive gluten monitoring.

Certain cereals like wheat, rye or barley contain gluten, a protein mixture that can trigger celiac disease (CD). To make gluten-free diets available for affected individuals the gluten content of foodstuff must be monitored. For this purpose, antibody-based assays exist which rely on the recognitio...

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Main Authors: Niels Röckendorf, Barbara Meckelein, Katharina A Scherf, Kathrin Schalk, Peter Koehler, Andreas Frey
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5536345?pdf=render
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spelling doaj-6e84fb1fbad14e219ea819ca7e6ee7362020-11-24T22:05:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01127e018156610.1371/journal.pone.0181566Identification of novel antibody-reactive detection sites for comprehensive gluten monitoring.Niels RöckendorfBarbara MeckeleinKatharina A ScherfKathrin SchalkPeter KoehlerAndreas FreyCertain cereals like wheat, rye or barley contain gluten, a protein mixture that can trigger celiac disease (CD). To make gluten-free diets available for affected individuals the gluten content of foodstuff must be monitored. For this purpose, antibody-based assays exist which rely on the recognition of certain linear gluten sequence motifs. Yet, not all CD-active gluten constituents and fragments formed during food processing/fermentation may be covered by those tests. In this study, we therefore assayed the coverage of reportedly CD-active gluten components by currently available detection antibodies and determined the antibody-inducing capacity of wheat gluten constituents in order to provide novel diagnostic targets for comprehensive gluten quantitation. Immunizations of outbred mice with purified gliadins and glutenins were conducted and the linear target recognition profile of the sera was recorded using synthetic peptide arrays that covered the sequence space of gluten constituents present in those preparations. The resulting murine immunorecognition profile of gluten demonstrated that further linear binding sites beyond those recognized by the monoclonal antibodies α20, R5 and G12 exist and may be exploitable as diagnostic targets. We conclude that the safety of foodstuffs for CD patients can be further improved by complementing current tests with antibodies directed against additional CD-active gluten components. Currently unrepresented linear gluten detection sites in glutenins and α-gliadins suggest sequences QQQYPS, PQQSFP, QPGQGQQG and QQPPFS as novel targets for antibody generation.http://europepmc.org/articles/PMC5536345?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Niels Röckendorf
Barbara Meckelein
Katharina A Scherf
Kathrin Schalk
Peter Koehler
Andreas Frey
spellingShingle Niels Röckendorf
Barbara Meckelein
Katharina A Scherf
Kathrin Schalk
Peter Koehler
Andreas Frey
Identification of novel antibody-reactive detection sites for comprehensive gluten monitoring.
PLoS ONE
author_facet Niels Röckendorf
Barbara Meckelein
Katharina A Scherf
Kathrin Schalk
Peter Koehler
Andreas Frey
author_sort Niels Röckendorf
title Identification of novel antibody-reactive detection sites for comprehensive gluten monitoring.
title_short Identification of novel antibody-reactive detection sites for comprehensive gluten monitoring.
title_full Identification of novel antibody-reactive detection sites for comprehensive gluten monitoring.
title_fullStr Identification of novel antibody-reactive detection sites for comprehensive gluten monitoring.
title_full_unstemmed Identification of novel antibody-reactive detection sites for comprehensive gluten monitoring.
title_sort identification of novel antibody-reactive detection sites for comprehensive gluten monitoring.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2017-01-01
description Certain cereals like wheat, rye or barley contain gluten, a protein mixture that can trigger celiac disease (CD). To make gluten-free diets available for affected individuals the gluten content of foodstuff must be monitored. For this purpose, antibody-based assays exist which rely on the recognition of certain linear gluten sequence motifs. Yet, not all CD-active gluten constituents and fragments formed during food processing/fermentation may be covered by those tests. In this study, we therefore assayed the coverage of reportedly CD-active gluten components by currently available detection antibodies and determined the antibody-inducing capacity of wheat gluten constituents in order to provide novel diagnostic targets for comprehensive gluten quantitation. Immunizations of outbred mice with purified gliadins and glutenins were conducted and the linear target recognition profile of the sera was recorded using synthetic peptide arrays that covered the sequence space of gluten constituents present in those preparations. The resulting murine immunorecognition profile of gluten demonstrated that further linear binding sites beyond those recognized by the monoclonal antibodies α20, R5 and G12 exist and may be exploitable as diagnostic targets. We conclude that the safety of foodstuffs for CD patients can be further improved by complementing current tests with antibodies directed against additional CD-active gluten components. Currently unrepresented linear gluten detection sites in glutenins and α-gliadins suggest sequences QQQYPS, PQQSFP, QPGQGQQG and QQPPFS as novel targets for antibody generation.
url http://europepmc.org/articles/PMC5536345?pdf=render
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AT kathrinschalk identificationofnovelantibodyreactivedetectionsitesforcomprehensiveglutenmonitoring
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