Isolation of dihydroflavonol 4-reductase cDNA clones from Angelonia x angustifolia and heterologous expression as GST fusion protein in Escherichia coli.

Isolation of dihydroflavonol 4-reductase cDNA clones from Angelonia x angustifolia and heterologous expression as GST fusion protein in Escherichia coli.

Blue Angelonia × angustifolia flowers can show spontaneous mutations resulting in white/blue and white flower colourations. In such a white line, a loss of dihydroflavonol 4-reductase (DFR) activity was observed whereas chalcone synthase and flavanone 3-hydroxylase activity remained unchanged. Thus,...

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Bibliographic Details
Main Authors: Christian Gosch, Karthik Mudigere Nagesh, Jana Thill, Silvija Miosic, Sylvia Plaschil, Malvina Milosevic, Klaus Olbricht, Shaghef Ejaz, Annette Rompel, Karl Stich, Heidi Halbwirth
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4169556?pdf=render

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http://europepmc.org/articles/PMC4169556?pdf=render

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