Can Targeting Non-Contiguous V-Regions With Paired-End Sequencing Improve 16S rRNA-Based Taxonomic Resolution of Microbiomes?: An In Silico Evaluation

Can Targeting Non-Contiguous V-Regions With Paired-End Sequencing Improve 16S rRNA-Based Taxonomic Resolution of Microbiomes?: An In Silico Evaluation

Background: Next-generation sequencing (NGS) technologies have enabled probing of microbial diversity in different environmental niches with unprecedented sequencing depth. However, due to read-length limitations of popular NGS technologies, 16S amplicon sequencing-based microbiome studies rely on t...

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Bibliographic Details
Main Authors: Nishal Kumar Pinna, Anirban Dutta, Mohammed Monzoorul Haque, Sharmila S. Mande
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-07-01
Series:Frontiers in Genetics
Subjects:
metagenomics 16S
paired-end sequencing
taxonomic profiling
microbiome analysis
amplicon sequencing
Online Access:https://www.frontiersin.org/article/10.3389/fgene.2019.00653/full

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