Sodium-calcium exchanger mediates sensory-evoked glial calcium transients in the developing retinotectal system

Summary: Various types of sensory stimuli have been shown to induce Ca2+ elevations in glia. However, a mechanistic understanding of the signaling pathways mediating sensory-evoked activity in glia in intact animals is still emerging. During early development of the Xenopus laevis visual system, rad...

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Main Authors: Nicholas J. Benfey, Vanessa J. Li, Anne Schohl, Edward S. Ruthazer
Format: Article
Language:English
Published: Elsevier 2021-10-01
Series:Cell Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124721012511
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spelling doaj-6e545cd321604089b693a19cd5add0432021-10-07T04:24:57ZengElsevierCell Reports2211-12472021-10-01371109791Sodium-calcium exchanger mediates sensory-evoked glial calcium transients in the developing retinotectal systemNicholas J. Benfey0Vanessa J. Li1Anne Schohl2Edward S. Ruthazer3Montreal Neurological Institute-Hospital, Department of Neurology and Neurosurgery, McGill University, Montréal, Québec, H3A 2B4 CanadaMontreal Neurological Institute-Hospital, Department of Neurology and Neurosurgery, McGill University, Montréal, Québec, H3A 2B4 CanadaMontreal Neurological Institute-Hospital, Department of Neurology and Neurosurgery, McGill University, Montréal, Québec, H3A 2B4 CanadaMontreal Neurological Institute-Hospital, Department of Neurology and Neurosurgery, McGill University, Montréal, Québec, H3A 2B4 Canada; Corresponding authorSummary: Various types of sensory stimuli have been shown to induce Ca2+ elevations in glia. However, a mechanistic understanding of the signaling pathways mediating sensory-evoked activity in glia in intact animals is still emerging. During early development of the Xenopus laevis visual system, radial astrocytes in the optic tectum are highly responsive to sensory stimulation. Ca2+ transients occur spontaneously in radial astrocytes at rest and are abolished by silencing neuronal activity with tetrodotoxin. Visual stimulation drives temporally correlated increases in the activity patterns of neighboring radial astrocytes. Following blockade of all glutamate receptors (gluRs), visually evoked Ca2+ activity in radial astrocytes persists, while neuronal activity is suppressed. The additional blockade of either glu transporters or sodium-calcium exchangers (NCX) abolishes visually evoked responses in glia. Finally, we demonstrate that blockade of NCX alone is sufficient to prevent visually evoked responses in radial astrocytes, highlighting a pivotal role for NCX in glia during development.http://www.sciencedirect.com/science/article/pii/S2211124721012511astrocyteradial glianeuron-glia interactionsXenopusglutamate transportercalcium imaging
collection DOAJ
language English
format Article
sources DOAJ
author Nicholas J. Benfey
Vanessa J. Li
Anne Schohl
Edward S. Ruthazer
spellingShingle Nicholas J. Benfey
Vanessa J. Li
Anne Schohl
Edward S. Ruthazer
Sodium-calcium exchanger mediates sensory-evoked glial calcium transients in the developing retinotectal system
Cell Reports
astrocyte
radial glia
neuron-glia interactions
Xenopus
glutamate transporter
calcium imaging
author_facet Nicholas J. Benfey
Vanessa J. Li
Anne Schohl
Edward S. Ruthazer
author_sort Nicholas J. Benfey
title Sodium-calcium exchanger mediates sensory-evoked glial calcium transients in the developing retinotectal system
title_short Sodium-calcium exchanger mediates sensory-evoked glial calcium transients in the developing retinotectal system
title_full Sodium-calcium exchanger mediates sensory-evoked glial calcium transients in the developing retinotectal system
title_fullStr Sodium-calcium exchanger mediates sensory-evoked glial calcium transients in the developing retinotectal system
title_full_unstemmed Sodium-calcium exchanger mediates sensory-evoked glial calcium transients in the developing retinotectal system
title_sort sodium-calcium exchanger mediates sensory-evoked glial calcium transients in the developing retinotectal system
publisher Elsevier
series Cell Reports
issn 2211-1247
publishDate 2021-10-01
description Summary: Various types of sensory stimuli have been shown to induce Ca2+ elevations in glia. However, a mechanistic understanding of the signaling pathways mediating sensory-evoked activity in glia in intact animals is still emerging. During early development of the Xenopus laevis visual system, radial astrocytes in the optic tectum are highly responsive to sensory stimulation. Ca2+ transients occur spontaneously in radial astrocytes at rest and are abolished by silencing neuronal activity with tetrodotoxin. Visual stimulation drives temporally correlated increases in the activity patterns of neighboring radial astrocytes. Following blockade of all glutamate receptors (gluRs), visually evoked Ca2+ activity in radial astrocytes persists, while neuronal activity is suppressed. The additional blockade of either glu transporters or sodium-calcium exchangers (NCX) abolishes visually evoked responses in glia. Finally, we demonstrate that blockade of NCX alone is sufficient to prevent visually evoked responses in radial astrocytes, highlighting a pivotal role for NCX in glia during development.
topic astrocyte
radial glia
neuron-glia interactions
Xenopus
glutamate transporter
calcium imaging
url http://www.sciencedirect.com/science/article/pii/S2211124721012511
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