DETERMINATION OF LEPTIN EXPRESSION IN BEEF CATTLE BLOOD SAMPLES USED BY RTQ PCR

DETERMINATION OF LEPTIN EXPRESSION IN BEEF CATTLE BLOOD SAMPLES USED BY RTQ PCR

The aim of our study was to detect the presence and concentration of leptin in different breeds of cattle by PCR and Real time PCR method. Blood of different breeds of bulls was used as biological material in our experiments: Slovak pied cattle (10 samples), Blondaquitane × Pinzgau breed (10 samples...

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Main Authors: Miroslava Kačániová, Juraj Čuboň, Peter Haščík, Lukáš Hleba, Simona Kunová
Format: Article
Language:English
Published: Slovak University of Agriculture 2011-08-01
Series:Journal of Microbiology, Biotechnology and Food Sciences
Subjects:
Leptin concentration
beef cattle
leptin gene
polymerase chain reaction
Online Access:http://www.jmbfs.org/wp-content/uploads/2011/09/JMBFS_0002_Kunová-et-al.-2011.pdf
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spelling doaj-6e52037d5faa4f688936ccce24f8b33e2020-11-25T01:14:46ZengSlovak University of AgricultureJournal of Microbiology, Biotechnology and Food Sciences1338-51782011-08-01112138DETERMINATION OF LEPTIN EXPRESSION IN BEEF CATTLE BLOOD SAMPLES USED BY RTQ PCRMiroslava KačániováJuraj ČuboňPeter HaščíkLukáš HlebaSimona KunováThe aim of our study was to detect the presence and concentration of leptin in different breeds of cattle by PCR and Real time PCR method. Blood of different breeds of bulls was used as biological material in our experiments: Slovak pied cattle (10 samples), Blondaquitane × Pinzgau breed (10 samples) and Holstein breed (10 samples). The presence of leptin was detected in all samples based on the results of molecular-genetic detection of leptin gene. The average concentration of leptinin 30 samples of beef cattle was 22.1477 μg.μl-1. Differences in leptin concentrations were statistically significant between Holstein breed and Slovak pied cattle and between Slovak pied cattle and Blondaquitane × Pinzgau breed. http://www.jmbfs.org/wp-content/uploads/2011/09/JMBFS_0002_Kunová-et-al.-2011.pdfLeptin concentrationbeef cattleleptin genepolymerase chain reaction
collection DOAJ
language English
format Article
sources DOAJ
author Miroslava Kačániová
Juraj Čuboň
Peter Haščík
Lukáš Hleba
Simona Kunová
spellingShingle Miroslava Kačániová
Juraj Čuboň
Peter Haščík
Lukáš Hleba
Simona Kunová
DETERMINATION OF LEPTIN EXPRESSION IN BEEF CATTLE BLOOD SAMPLES USED BY RTQ PCR
Journal of Microbiology, Biotechnology and Food Sciences
Leptin concentration
beef cattle
leptin gene
polymerase chain reaction
author_facet Miroslava Kačániová
Juraj Čuboň
Peter Haščík
Lukáš Hleba
Simona Kunová
author_sort Miroslava Kačániová
title DETERMINATION OF LEPTIN EXPRESSION IN BEEF CATTLE BLOOD SAMPLES USED BY RTQ PCR
title_short DETERMINATION OF LEPTIN EXPRESSION IN BEEF CATTLE BLOOD SAMPLES USED BY RTQ PCR
title_full DETERMINATION OF LEPTIN EXPRESSION IN BEEF CATTLE BLOOD SAMPLES USED BY RTQ PCR
title_fullStr DETERMINATION OF LEPTIN EXPRESSION IN BEEF CATTLE BLOOD SAMPLES USED BY RTQ PCR
title_full_unstemmed DETERMINATION OF LEPTIN EXPRESSION IN BEEF CATTLE BLOOD SAMPLES USED BY RTQ PCR
title_sort determination of leptin expression in beef cattle blood samples used by rtq pcr
publisher Slovak University of Agriculture
series Journal of Microbiology, Biotechnology and Food Sciences
issn 1338-5178
publishDate 2011-08-01
description The aim of our study was to detect the presence and concentration of leptin in different breeds of cattle by PCR and Real time PCR method. Blood of different breeds of bulls was used as biological material in our experiments: Slovak pied cattle (10 samples), Blondaquitane × Pinzgau breed (10 samples) and Holstein breed (10 samples). The presence of leptin was detected in all samples based on the results of molecular-genetic detection of leptin gene. The average concentration of leptinin 30 samples of beef cattle was 22.1477 μg.μl-1. Differences in leptin concentrations were statistically significant between Holstein breed and Slovak pied cattle and between Slovak pied cattle and Blondaquitane × Pinzgau breed.
topic Leptin concentration
beef cattle
leptin gene
polymerase chain reaction
url http://www.jmbfs.org/wp-content/uploads/2011/09/JMBFS_0002_Kunová-et-al.-2011.pdf
work_keys_str_mv AT miroslavakacaniova determinationofleptinexpressioninbeefcattlebloodsamplesusedbyrtqpcr
AT jurajcubon determinationofleptinexpressioninbeefcattlebloodsamplesusedbyrtqpcr
AT peterhascik determinationofleptinexpressioninbeefcattlebloodsamplesusedbyrtqpcr
AT lukashleba determinationofleptinexpressioninbeefcattlebloodsamplesusedbyrtqpcr
AT simonakunova determinationofleptinexpressioninbeefcattlebloodsamplesusedbyrtqpcr
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