Detection of foot-and-mouth disease virus rna by reverse transcription loop-mediated isothermal amplification

• Main Authors: Chen Hao-tai, Zhang Jie, Liu Yong-sheng, Liu Xiang-tao
• Format: Article
• Language: English
• Published: BMC 2011-11-01
• Series: Virology Journal
• Subjects: Foot-and-mouth disease virus; Detection; Reverse transcription loop-mediated isothermal amplification; Sensitivity; Specificity
• Online Access: http://www.virologyj.com/content/8/1/510
• ISSN: 1743-422X

<p>Abstract</p> <p>A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for foot-and-mouth disease virus (FMDV) RNA. The amplification was able to finish in 45 min under isothermal condition at 64°C by employing a set of four primers targeting FMDV 2B. The assay showed higher sensitivity than RT-PCR. No cross reactivity was observed from other RNA viruses including classical swine fever virus, swine vesicular disease, porcine reproductive and respiratory syndrome virus, Japanese encephalitis virus. Furthermore, the assay correctly detected 84 FMDV positive samples but not 65 FMDV negative specimens. The result indicated the potential usefulness of the technique as a simple and rapid procedure for the detection of FMDV infection.</p>