Anti-Giardial Activity of Chloroformic Extract of Tanacetum parthenium and Artemisia annua in vitro

Background: Giardiasis is a one of the most prevalent intestinal parasitic diseases in human, treatment of this disease through medicinal plants is very important since parasite resistance to chemical drugs exists. Thus, in this study, the in vitro anti-giardial activity of chloroformic extract of T...

Full description

Bibliographic Details
Main Authors: Shirzad Gholami, Mohammad Azadbakht, Hajar Ziaei Hezarjaribi, Bahman Rahimi-Esboei
Format: Article
Language:English
Published: Mazandaran University of Medical Sciences and Health Services 2014-02-01
Series:Research in Molecular Medicine
Subjects:
Online Access:http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-21&slc_lang=en&sid=1
id doaj-6de3052fdc19474d8dc5b27896b8e236
record_format Article
spelling doaj-6de3052fdc19474d8dc5b27896b8e2362020-11-25T03:54:02ZengMazandaran University of Medical Sciences and Health ServicesResearch in Molecular Medicine2322-13482322-133X2014-02-01214651Anti-Giardial Activity of Chloroformic Extract of Tanacetum parthenium and Artemisia annua in vitroShirzad Gholami0Mohammad Azadbakht1Hajar Ziaei Hezarjaribi2Bahman Rahimi-Esboei3 Background: Giardiasis is a one of the most prevalent intestinal parasitic diseases in human, treatment of this disease through medicinal plants is very important since parasite resistance to chemical drugs exists. Thus, in this study, the in vitro anti-giardial activity of chloroformic extract of Tanacetum parthenium and Artemisia annua on cyst and trophozoite of Giardia lamblia were separately investigated.Materials and Methods: In this experimental study chloroformic extracts of Artemisia annua and Tanacetum parthenium on cyst and trophozoite of G. lamblia in vitro were prepared in 1, 10, 50 and 100 mg∕ml concentrations for 5, 10, 30, 60 and 180 min. Purified cysts were used for encystations and culture in TYI-S-33 medium. Then, 2 ml of each solution was placed in test tubes, to which 10,000 washed cysts and trophozoites were added. The contents of the tubes were gently mixed and incubated. The percentages of dead parasites were determined by counting 500 cysts. Non treated parasites were considered a control group in each experiment and the viability of the parasites checked with Eeosin staining and statistical analysis were done. Results: The results showed that chloroformic extracts of A. annua at 100 mg/ml concentration affected on Gardia cyst 86% and 100% trophozoite after 1 hour. T. parthenium at 50mg/ml concentration killed cysts (83%) and trophozite (100%) after 1 hour, respectively. T. parthenium chloroformic extract had a better effect on cyst and trophozoite of Giardia at 50 mg/ml after 1 hour exposure than A. annua extract. Conclusion: According to this study, A. annua and T. parthenium chlorofomic extracts could be considered as a more effective anti-giardial agent. Chloroformic extract of T. parthenium was also shown the anti-giardial activity compared with A. annua and control groups at all exposure times. Therefore, in the future research using these plants are recommended against Giardia in low concentration in the in vivo, also to find fractions of the pharmacological effects of these plants.http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-21&slc_lang=en&sid=1Giardia lambelia Chlorofomic extract Artemisia annua Tanacetum parthenium Cyst Trophozoite
collection DOAJ
language English
format Article
sources DOAJ
author Shirzad Gholami
Mohammad Azadbakht
Hajar Ziaei Hezarjaribi
Bahman Rahimi-Esboei
spellingShingle Shirzad Gholami
Mohammad Azadbakht
Hajar Ziaei Hezarjaribi
Bahman Rahimi-Esboei
Anti-Giardial Activity of Chloroformic Extract of Tanacetum parthenium and Artemisia annua in vitro
Research in Molecular Medicine
Giardia lambelia
Chlorofomic extract
Artemisia annua
Tanacetum parthenium
Cyst
Trophozoite
author_facet Shirzad Gholami
Mohammad Azadbakht
Hajar Ziaei Hezarjaribi
Bahman Rahimi-Esboei
author_sort Shirzad Gholami
title Anti-Giardial Activity of Chloroformic Extract of Tanacetum parthenium and Artemisia annua in vitro
title_short Anti-Giardial Activity of Chloroformic Extract of Tanacetum parthenium and Artemisia annua in vitro
title_full Anti-Giardial Activity of Chloroformic Extract of Tanacetum parthenium and Artemisia annua in vitro
title_fullStr Anti-Giardial Activity of Chloroformic Extract of Tanacetum parthenium and Artemisia annua in vitro
title_full_unstemmed Anti-Giardial Activity of Chloroformic Extract of Tanacetum parthenium and Artemisia annua in vitro
title_sort anti-giardial activity of chloroformic extract of tanacetum parthenium and artemisia annua in vitro
publisher Mazandaran University of Medical Sciences and Health Services
series Research in Molecular Medicine
issn 2322-1348
2322-133X
publishDate 2014-02-01
description Background: Giardiasis is a one of the most prevalent intestinal parasitic diseases in human, treatment of this disease through medicinal plants is very important since parasite resistance to chemical drugs exists. Thus, in this study, the in vitro anti-giardial activity of chloroformic extract of Tanacetum parthenium and Artemisia annua on cyst and trophozoite of Giardia lamblia were separately investigated.Materials and Methods: In this experimental study chloroformic extracts of Artemisia annua and Tanacetum parthenium on cyst and trophozoite of G. lamblia in vitro were prepared in 1, 10, 50 and 100 mg∕ml concentrations for 5, 10, 30, 60 and 180 min. Purified cysts were used for encystations and culture in TYI-S-33 medium. Then, 2 ml of each solution was placed in test tubes, to which 10,000 washed cysts and trophozoites were added. The contents of the tubes were gently mixed and incubated. The percentages of dead parasites were determined by counting 500 cysts. Non treated parasites were considered a control group in each experiment and the viability of the parasites checked with Eeosin staining and statistical analysis were done. Results: The results showed that chloroformic extracts of A. annua at 100 mg/ml concentration affected on Gardia cyst 86% and 100% trophozoite after 1 hour. T. parthenium at 50mg/ml concentration killed cysts (83%) and trophozite (100%) after 1 hour, respectively. T. parthenium chloroformic extract had a better effect on cyst and trophozoite of Giardia at 50 mg/ml after 1 hour exposure than A. annua extract. Conclusion: According to this study, A. annua and T. parthenium chlorofomic extracts could be considered as a more effective anti-giardial agent. Chloroformic extract of T. parthenium was also shown the anti-giardial activity compared with A. annua and control groups at all exposure times. Therefore, in the future research using these plants are recommended against Giardia in low concentration in the in vivo, also to find fractions of the pharmacological effects of these plants.
topic Giardia lambelia
Chlorofomic extract
Artemisia annua
Tanacetum parthenium
Cyst
Trophozoite
url http://rmm.mazums.ac.ir/browse.php?a_code=A-10-26-21&slc_lang=en&sid=1
work_keys_str_mv AT shirzadgholami antigiardialactivityofchloroformicextractoftanacetumpartheniumandartemisiaannuainvitro
AT mohammadazadbakht antigiardialactivityofchloroformicextractoftanacetumpartheniumandartemisiaannuainvitro
AT hajarziaeihezarjaribi antigiardialactivityofchloroformicextractoftanacetumpartheniumandartemisiaannuainvitro
AT bahmanrahimiesboei antigiardialactivityofchloroformicextractoftanacetumpartheniumandartemisiaannuainvitro
_version_ 1724475202320990208