Expression and Epigenetic Change of the AR and FSHR Genes in the Granulosa Cells of Endometriosis Patients

• Main Authors: Mika Hayashi, Yoshiki Yamashita, Atsushi Hayashi, Yoko Yoshida, Sachiko Kawabe, Masami Hayashi, Yoshito Terai, Hideki Kamegai, Masahide Ohmichi
• Format: Article
• Language: English
• Published: SAGE Publishing 2012-01-01
• Series: Genetics and Epigenetics
• Online Access: https://doi.org/10.4137/GEG.S9877

Background Endometriosis is one of the most common gynecological diseases associated with infertility. Endometriosis may affect the androgen receptor (AR) mRNA expression in human granulosa cells and the methylation of the promoter region of AR. We investigated 28 patients with endometriosis and 47 subjects without endometriosis undertaking IVF treatment. Methods Granulosa cells were obtained from 28 patients with endometriosis and 47 subjects without endometriosis as a control. Expressions of AR and FSHR mRNA were then evaluated by OneStep real-time PCR analysis, and the level of methylation of the promoter region was qualified by methylation-specified PCR (MSP). Results The expression of AR mRNA in the endometriosis group was statistically lower than that in the control group. As well, FSHR mRNA expression in the control group showed a positive correlation with AR mRNA expression; however, there was no such correlation in endometriosis patients. In the control group, AR mRNA expression was statistically higher in pregnant subjects compared with non-pregnant subjects; however, in the endometriosis group, no significant difference was identified. The promoter of AR was heavily methylated in all endometriosis cases; however, only 5 (45.4%) were methylated in the control group. Conclusion Lower AR mRNA expression and methylation of the AR promoter region might affect the expression of AR and FSHR the presence of endometriosis, thus leading to a disturbance in the regulation of AR and FSHR.