Generation of induced pluripotent stem cell line (ZZUi0024-A) from a 51-year-old patient with APP gene mutation in Alzheimer’ s disease

Generation of induced pluripotent stem cell line (ZZUi0024-A) from a 51-year-old patient with APP gene mutation in Alzheimer’ s disease

Alzheimer’s disease (AD) is a neurodegenerative disease that manifests mainly as cognitive, behavioral, and neuropsychiatric changes and impairs social functions and activities of daily living. The β-amyloid precursor protein (APP) gene is one of the most common pathogenic genes associated with AD....

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Main Authors: Zhuoya Wang, Huifang Sun, Qi Zhang, Jing Yang, Yutao Liu, Han Liu, Changhe Shi, Yanlin Wang, Yuming Xu
Format: Article
Language:English
Published: Elsevier 2021-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121001136
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spelling doaj-6dd18aa43d8640ecb77b2e365d13514a2021-05-30T04:41:15ZengElsevierStem Cell Research1873-50612021-05-0153102267Generation of induced pluripotent stem cell line (ZZUi0024-A) from a 51-year-old patient with APP gene mutation in Alzheimer’ s diseaseZhuoya Wang0Huifang Sun1Qi Zhang2Jing Yang3Yutao Liu4Han Liu5Changhe Shi6Yanlin Wang7Yuming Xu8Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, China; Academy of Medical Science, Zhengzhou University, Zhengzhou, Henan 450001, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, China; Academy of Medical Science, Zhengzhou University, Zhengzhou, Henan 450001, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, China; Corresponding authors.Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450052, China; Corresponding authors.Alzheimer’s disease (AD) is a neurodegenerative disease that manifests mainly as cognitive, behavioral, and neuropsychiatric changes and impairs social functions and activities of daily living. The β-amyloid precursor protein (APP) gene is one of the most common pathogenic genes associated with AD. We isolated dermal fibroblasts from a 51-year-old woman with an APP gene mutation (c.1756G > A). The induced pluripotent stem cells (iPSCs) were successfully constructed by transferring the reprogramming plasmids expressing OCT3/4, SOX2, KLF4, LIN28, and L-MYC. The generated iPSC line was pluripotent, as verified by immunofluorescence, flow cytometry, and teratoma formation test. The iPSC line will have broad prospects in drug screening, cell transplantation, and gene therapy.http://www.sciencedirect.com/science/article/pii/S1873506121001136
collection DOAJ
language English
format Article
sources DOAJ
author Zhuoya Wang
Huifang Sun
Qi Zhang
Jing Yang
Yutao Liu
Han Liu
Changhe Shi
Yanlin Wang
Yuming Xu
spellingShingle Zhuoya Wang
Huifang Sun
Qi Zhang
Jing Yang
Yutao Liu
Han Liu
Changhe Shi
Yanlin Wang
Yuming Xu
Generation of induced pluripotent stem cell line (ZZUi0024-A) from a 51-year-old patient with APP gene mutation in Alzheimer’ s disease
Stem Cell Research
author_facet Zhuoya Wang
Huifang Sun
Qi Zhang
Jing Yang
Yutao Liu
Han Liu
Changhe Shi
Yanlin Wang
Yuming Xu
author_sort Zhuoya Wang
title Generation of induced pluripotent stem cell line (ZZUi0024-A) from a 51-year-old patient with APP gene mutation in Alzheimer’ s disease
title_short Generation of induced pluripotent stem cell line (ZZUi0024-A) from a 51-year-old patient with APP gene mutation in Alzheimer’ s disease
title_full Generation of induced pluripotent stem cell line (ZZUi0024-A) from a 51-year-old patient with APP gene mutation in Alzheimer’ s disease
title_fullStr Generation of induced pluripotent stem cell line (ZZUi0024-A) from a 51-year-old patient with APP gene mutation in Alzheimer’ s disease
title_full_unstemmed Generation of induced pluripotent stem cell line (ZZUi0024-A) from a 51-year-old patient with APP gene mutation in Alzheimer’ s disease
title_sort generation of induced pluripotent stem cell line (zzui0024-a) from a 51-year-old patient with app gene mutation in alzheimer’ s disease
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2021-05-01
description Alzheimer’s disease (AD) is a neurodegenerative disease that manifests mainly as cognitive, behavioral, and neuropsychiatric changes and impairs social functions and activities of daily living. The β-amyloid precursor protein (APP) gene is one of the most common pathogenic genes associated with AD. We isolated dermal fibroblasts from a 51-year-old woman with an APP gene mutation (c.1756G > A). The induced pluripotent stem cells (iPSCs) were successfully constructed by transferring the reprogramming plasmids expressing OCT3/4, SOX2, KLF4, LIN28, and L-MYC. The generated iPSC line was pluripotent, as verified by immunofluorescence, flow cytometry, and teratoma formation test. The iPSC line will have broad prospects in drug screening, cell transplantation, and gene therapy.
url http://www.sciencedirect.com/science/article/pii/S1873506121001136
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