IMPACT OF BACTERIAL QUORUM SENSING SYSTEM ON CHANGES OF ORGANOLEPTIC MARKERS OF STORAGE CABBAGE

• Main Authors: Kamila Myszka, Mariola Olkowicz, Elżbieta Radziejewska-Kubzdela, Agnieszka K. Olejnik-Schmidt, Katarzyna Czaczyk
• Format: Article
• Language: English
• Published: Slovak University of Agriculture 2015-04-01
• Series: Journal of Microbiology, Biotechnology and Food Sciences
• Subjects: Enterobacteriaceae; quorum sensing; acylated homoserine lactones; storage cabbage; microbiological spoilage
• Online Access: http://www.jmbfs.org/wp-content/uploads/2015/04/jmbfs-0506-myszka.pdf

The regulation of phenotypes of vegetable-associated bacteria can be mediated through the production of acylated homoserine lactones (AHLs). This knowledge is essential for successful control of bacterial diseases of vegetable. The purpose of these investigations was to define the AHL patterns of gram-negative bacteria presented in storage cabbage by LC/MS technique. The phenomenon of regulating the pectinolytic activity and the exopolysaccharide (EPS) production by AHLs that are associated with microbial spoilage of cabbage was also examined. Among 100 strains isolated from storage cabbage, 47 isolates produced AHLs. The results of the 16S rDNA sequence analysis indicated that selected microflora was highly closely related to Pantoea agglomerans, Rahnella aquatilis, Pseudomonas fluorescens, Pseudomonas syringae, and Pseudomonas cedrina (approximately 98%-99% confidence). The chemical nature of AHLs produced by selected microflora differ from species to species. The pattern of AHLs of Ps. cedrina consisting of C8-HSL, 3-oxo-C10-HSL, and 3-hydroxy-C14-HSL, has not been previously reported. The present study demonstrates that bacterial spoilage of storage cabbage is influenced by quorum sensing. Application of furanone C-30 that acts as quorum sensing inhibitor, caused the significant reduction in the production of EPS and pectinolytic enzymes by examined bacteria.